USP14 Antibody (28C4)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26262
Recombinant monoclonal antibody expressed in HEK293F cells
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 28C4
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthesized peptide derived from Human USP14 [UniProt P54578]
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for USP14 Antibody (28C4)
Western Blot: USP14 Antibody (28C4) [NBP3-26262] -
Western Blot: USP14 Antibody (28C4) [NBP3-26262] - Positive Western Blot detected in: PC3 whole cell lysate, Ntera-2 whole cell lysate, MCF-7 whole cell lysate, Hela whole cell lysate, HepG2 whole cell lysate, A549 whole cell lysate, U251 whole cell lysate.All lanes: USP14 Antibody at 1: 2000
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 57, 53, 55 kDa
Observed band size: 55-72 kDa
Immunocytochemistry/Immunofluorescence: USP14 Antibody (28C4) [NBP3-26262] -
Immunocytochemistry/Immunofluorescence: USP14 Antibody (28C4) [NBP3-26262] - Staining of PC-3 cell with USP14 Antibody (28C4) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 517-conjugated Goat Anti-Rabbit IgG (H+L).Applications for USP14 Antibody (28C4)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50-1:200
Western Blot
1:500-1:2000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, pH 7.4, 150mM NaCl and 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -70C. Avoid freeze-thaw cycles.
Background: USP14
Long Name
Ubiquitin Specific Protease 14
Alternate Names
TGT, Ubiquitin Thioesterase 14, Ubp6
Gene Symbol
USP14
Additional USP14 Products
Product Documents for USP14 Antibody (28C4)
Product Specific Notices for USP14 Antibody (28C4)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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