USP15 Antibody

Western Blot: USP15 Antibody [NB110-40690] -

Western Blot: USP15 Antibody [NB110-40690] - USP15 knockdown leads to decreased HECTD1 protein levels(a) USP15 knockdown with three siRNAs in LN-229 & (b) two siRNAs in LN-428. Image collected & cropped by CiteAb from the following publication (https://www.oncotarget.com/lookup/doi/10.18632/oncotarget.22798), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: USP15 Antibody [NB110-40690] -

Western Blot: USP15 Antibody [NB110-40690] - USP15 de-ubiquitinates & stabilizes HECTD1 in LN-229The de-ubiquitination assay was performed in cells transfected with an expression plasmid for HA-Ubiquitin that 40 hours after transfection were treated for 8 hours with the proteasome inhibitor MG132 (5μM). The cells were lyzed & used for IP using an antibody against the endogenous HECTD1, or an AB against rabbit IgG in the negative control. The immunoprecipitated proteins were then analyzed by Western blot using an anti HA antibody to detect ubiquitination. (a) LN-229 cells stably expressing USP15, the mutant USP15C/S, or the empty vector control, respectively. Lanes 1 to 3 correspond to the input controls. The USP15 wt overexpressing LN-229 cells show lower ubiquitination levels of HECTD1 than the vector control. The mutant USP15-C/S overexpressing cells displayed the highest ubiquitination of HECTD1. (b) De-ubiquitination assay in LN-229 depleted for endogenous USP15 by siRNA against USP15 or the respective si-control. LN-229 cells depleted for endogenous USP15 display higher ubiquitination of HECTD1 than the sicontrol cells. Image collected & cropped by CiteAb from the following publication (https://www.oncotarget.com/lookup/doi/10.18632/oncotarget.22798), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: USP15 Antibody [NB110-40690] -

Western Blot: USP15 Antibody [NB110-40690] - Effect of USP15 on cell growth in LN-229(a) Structure of the wildtype USP15 construct with the V5 protein tag, & the respective catalytic mutant USP15C298S. (b) Protein expression by Western blot using an anti-V5 AB, loading control tubulin for the selected clones from LN-229 & LN-428 with stable expression of USP15, or USP15C298S (USPC/S), or empty vector controls. (c) Growth of USP15, or USP15C298S (USPC/S) overexpressing clones, & respective empty vector control (pIRES2/EGFP) followed over 5 days in culture. The error bars indicate standard deviations of triplicate samples. (d) Adhesion-independent growth in soft agar, representative images, & quantification of total colony numbers. Empty vector control, eV, black; USP15, blue; USP15C/S, green. Histogram shows data as mean +/-SD. Image collected & cropped by CiteAb from the following publication (https://www.oncotarget.com/lookup/doi/10.18632/oncotarget.22798), licensed under a CC-BY license. Not internally tested by Novus Biologicals.