USP2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-86883

Novus Biologicals, part of Bio-Techne
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NBP1-86883
NBP1-86883-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (92%), Rat (93%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all USP2 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: QGLAGLRNLGNTCFMNSILQCLSNTRELRDYCLQRLYMRDLHHGSNAHTALVEEFAKLIQTIWTSSPNDVVSPSEFKTQIQRYAPRFVGYNQQDAQEFLRFLLDGLHNEVNRVTLRPKSNPENLDHLP

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for USP2 Antibody

Western Blot: USP2 Antibody [NBP1-86883]

Western Blot: USP2 Antibody [NBP1-86883]

Western Blot: USP2 Antibody [NBP1-86883] - Analysis in control (vector only transfected HEK293T lysate) and USP2 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: USP2 Antibody [NBP1-86883]

Immunocytochemistry/ Immunofluorescence: USP2 Antibody [NBP1-86883]

Immunocytochemistry/Immunofluorescence: USP2 Antibody [NBP1-86883] - Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: USP2 Antibody [NBP1-86883]

Immunohistochemistry-Paraffin: USP2 Antibody [NBP1-86883]

Immunohistochemistry-Paraffin: USP2 Antibody [NBP1-86883] - Staining of human duodenum shows strong luminal membranous and cytoplasmic positivity in glandular cells.

Applications for USP2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200-1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: USP2

UBP, an ubiquitin-specific protease, is selectively up regulated in bone by the osteotropic agents PTH, PTHrP and PGE2 and possibly via the PKA/cAMP pathway. It is also thought to play a role in the recycling of ubiquitin by hydrolysis of branched poly-ubiquitin from linear poly-ubiquitin chains, production of free ubiquitin from linear poly-ubiquitin chains and from certain ribosomal ubiquitin fusion proteins.

Long Name

Ubiquitin-specific Protease 2

Alternate Names

UBP41, USP9

Gene Symbol

USP2

Additional USP2 Products

Product Documents for USP2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for USP2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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