USP3 Antibody (1H2)

Novus Biologicals, part of Bio-Techne | Catalog # H00009960-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 1H2

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all USP3 Antibodies »

Product Specifications

Immunogen

USP3 (NP_006528, 421 a.a. ~ 520 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. RNKVDTYVEFPLRGLDMKCYLLEPENSGPESCLYDLAAVVVHHGSGVGSGHYTAYATHEGRWFHFNDSTVTLTDEETVVKAKAYILFYVEHQAKAGSDKL

Specificity

USP3 (1H2)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for USP3 Antibody (1H2)

Western Blot: USP3 Antibody (1H2) [H00009960-M01]

Western Blot: USP3 Antibody (1H2) [H00009960-M01]

Western Blot: USP3 Antibody (1H2) [H00009960-M01] - Analysis of USP3 expression in transfected 293T cell line by USP3 monoclonal antibody (M01), clone 1H2.Lane 1: USP3 transfected lysate(58.9 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: USP3 Antibody (1H2) [H00009960-M01]

Immunocytochemistry/ Immunofluorescence: USP3 Antibody (1H2) [H00009960-M01]

Immunocytochemistry/Immunofluorescence: USP3 Antibody (1H2) [H00009960-M01] - Analysis of monoclonal antibody to USP3 on HeLa cell . Antibody concentration 10 ug/ml.
ELISA: USP3 Antibody (1H2) [H00009960-M01]

ELISA: USP3 Antibody (1H2) [H00009960-M01]

ELISA: USP3 Antibody (1H2) [H00009960-M01] - Detection limit for recombinant GST tagged USP3 is approximately 0.3ng/ml as a capture antibody.

Applications for USP3 Antibody (1H2)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against transfected lysate and recombinant protein for western blot. It has also been used for ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: USP3

Ubiquitin (Ub) is a highly conserved protein found ubiquitously in eukaryotic organisms. The conjugation of ubiquitin to proteins is an important means to regulate protein activity for many cellular processes by tagging them for degradation. Removal of Ub can rescue proteins from degradation. This is accomplished by the ubiquitin-specific processing protease (UBP) family of enzymes. Ubiquitin specific processing protease 3 (USP3) is a member of this family and is involved in the deubiquitination of histones H2A and H2B. Alternate names for USP3 include ubiquitin carboxyl-terminal hydrolase 3, ubiquitin thioesterase 3, ubiquitin-specific-processing protease 3, and deubiquitinating enzyme 3.

Long Name

Ubiquitin carboxyl-terminal hydrolase 3

Alternate Names

Deubiquitinating enzyme 3, EC 3.1.2.15, EC 3.4.19.12, MGC129878, MGC129879, SIH003, ubiquitin carboxyl-terminal hydrolase 3, ubiquitin specific peptidase 3, ubiquitin specific protease 3, ubiquitin thioesterase 3, Ubiquitin thiolesterase 3, Ubiquitin-specific-processing protease 3, UBP

Entrez Gene IDs

9960 (Human)

Gene Symbol

USP3

OMIM

604728 (Human)

UniProt

NP_006528 (Human)

Additional USP3 Products

Product Documents for USP3 Antibody (1H2)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for USP3 Antibody (1H2)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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