USP32 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-13509

Novus Biologicals, part of Bio-Techne
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NBP2-13509
NBP2-13509-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (90%), Rat (90%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all USP32 Antibodies »

Product Summary for USP32 Antibody

Immunogen

This antibody was developed against a recombinant protein corresponding to the amino acids: VVIEPSSVLNGGKYSFGTAAHPMEQVEDRIGSSLSYVNTTEEKFSDNISTASEASETAGSGFLYSATPGADVCFARQHNTSDNNNQCLLGANGNILLHLNPQKPGAIDNQPLVTQEPVKATSLTLEGGRLKRTPQLIHGRDYEMVPE

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for USP32 Antibody

Western Blot: USP32 Antibody [NBP2-13509]

Western Blot: USP32 Antibody [NBP2-13509]

Western Blot: USP32 Antibody [NBP2-13509] - Analysis in human cell line MCF-7.
Immunocytochemistry/ Immunofluorescence: USP32 Antibody [NBP2-13509]

Immunocytochemistry/ Immunofluorescence: USP32 Antibody [NBP2-13509]

Immunocytochemistry/Immunofluorescence: USP32 Antibody [NBP2-13509] - Staining of human cell line MCF7 shows localization to the Golgi apparatus. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: USP32 Antibody [NBP2-13509]

Immunohistochemistry-Paraffin: USP32 Antibody [NBP2-13509]

Immunohistochemistry-Paraffin: USP32 Antibody [NBP2-13509] - Staining of human testis shows moderate to strong cytoplasmic positivity in cells in seminiferous ducts.
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Applications for USP32 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: USP32

Ubiquitin-specific peptidase 32 (USP32) is a thioesterase enzyme that catalyzes the hydrolysis of ubiquitin from ubiquitin conjugates. USP32 has also been identified as an antigen recognized by autologous antibodies in patients with renal-cell carcinoma and as part of a chimeric gene, the Tre2 (USP6) oncogene, which resulted from a fusion between USP32 and TBC1D3.

Long Name

Ubiquitin Specific Protease 32

Alternate Names

NY-REN-60

Gene Symbol

USP32

Additional USP32 Products

Product Documents for USP32 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for USP32 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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