USP9x Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-48321

Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
NBP1-48321
NBP1-48321SS
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Predicted:

Rat (95%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.2 mg/ml

View all USP9x Antibodies »

Product Specifications

Immunogen

Genomic peptide made to an internal region of the human USP9x protein (within residues 1150-1300). [Swiss-Prot Q93008]

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: rat (95%) and zebrafish (80%).

Localization

Cytoplasm

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

270 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for USP9x Antibody

Western Blot: USP9x Antibody [NBP1-48321]

Western Blot: USP9x Antibody [NBP1-48321]

Western Blot: USP9x Antibody [NBP1-48321] - WT1 is a substrate of pUSP9X (serine 2563) in mitosis. Co-immunoprecipitation of FLAG-tagged WT1 with endogenous USP9X from HEK 293T cells (EV, expression vector). Cells were exposed to nocodazole, collected, lysed (WCE, whole cell extracts), and subjected to anti-FLAG immunoprecipitation before analysis by western blot. Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/s41467-020-15059-5), licensed under a CC-BY license.
Immunocytochemistry/ Immunofluorescence: USP9x Antibody [NBP1-48321]

Immunocytochemistry/ Immunofluorescence: USP9x Antibody [NBP1-48321]

Immunocytochemistry/Immunofluorescence: USP9x Antibody [NBP1-48321] - Immunocytochemical analysis of USP9X in NTERA-2 cells
Immunohistochemistry: USP9x Antibody [NBP1-48321]

Immunohistochemistry: USP9x Antibody [NBP1-48321]

Immunohistochemistry: USP9x Antibody [NBP1-48321] - IHC staining of Usp9x in brain tissue.
View 3 more images

Applications for USP9x Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:25-1:200

Immunohistochemistry

1:50

Immunohistochemistry-Paraffin

1:50

Western Blot

1:1000
Application Notes
This USP9x antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry paraffin embedded sections and Western blot, where a band is seen ~270 kDa. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reviewed Applications

Read 1 review rated 5 using NBP1-48321 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS, 0.1% BSA, and 30% Glycerol

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: USP9x

USP9x (ubiquitin-specific protease 9, X chromosome) is one of the largest deubiquitinases (DUBs) belonging to the Ub-specific protease family and implicates in processing of ubiquitin precursors as well as of ubiquitinated proteins, thereby playing a key regulatory role at protein turnover level by preventing degradation of proteins through conjugated ubiquitin removal. USP9X substrates include AF-6, beta-catenin, NUAK1, MARK4, ErbB2, EFA6, Smad4, Mcl1, ASK1, and survivin, and USP9X has been involved in several biological processes that include: protein trafficking and tight junction biogenesis in epithelial cells; regulation of the TGF-beta pathway; chromosome alignment and segregation; component of PEX5-mediated peroxisomal protein import pathway. Moreover, USP9X modulates the proteins levels of: alpha-synuclein, a protein involved in the pathogenesis of Parkinson disease; MCL1, an anti-apoptotic protein generally found overexpressed in cancers; some protein kinases involved in stress responses and cellular energy homeostasis; and some ubiquitin-ligases, such as MARCH7 and Itch.

Long Name

Ubiquitin-specific Protease 9, X-linked

Alternate Names

DFFRX, FAF, FAM

Entrez Gene IDs

8239 (Human); 22284 (Mouse)

Gene Symbol

USP9X

UniProt

Q93008 (Human), P70398 (Mouse)

Additional USP9x Products

Product Documents for USP9x Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for USP9x Antibody

Manufactured by Genomic Antibody Technology™. GAT FAQs

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.