UTP18 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-97492

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all UTP18 Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with E. coli-derived Human UTP18 fragment.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for UTP18 Antibody

Western Blot: UTP18 Antibody [NBP2-97492]

Western Blot: UTP18 Antibody [NBP2-97492]

Western Blot: UTP18 Antibody [NBP2-97492] - Anti-UTP18 rabbit polyclonal antibody at 1:500 dilution. Lane A: U-251 MG Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 62 kDa. Observed band size: 70 kDa
Immunocytochemistry/ Immunofluorescence: UTP18 Antibody [NBP2-97492]

Immunocytochemistry/ Immunofluorescence: UTP18 Antibody [NBP2-97492]

Immunocytochemistry/Immunofluorescence: UTP18 Antibody [NBP2-97492] - Immunofluorescence staining of UTP18 in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human UTP18 polyclonal antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.
Immunoprecipitation: UTP18 Antibody [NBP2-97492]

Immunoprecipitation: UTP18 Antibody [NBP2-97492]

Immunoprecipitation: UTP18 Antibody [NBP2-97492] - UTP18 was immunoprecipitated using: Lane A: 0.5 mg U-251MG Whole Cell Lysate 4 ul anti-UTP18 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-UTP18 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 62 kDa. Observed band size: 70 kDa

Applications for UTP18 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:500

Immunoprecipitation

5-10 uL/mg of lysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

PBS

Preservative

0.03% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: UTP18

UTP18/WDR50 is a component of the small-subunit (SSU) processome, a large ribonucleoprotein complex that participates in ribosomal RNA processing and ribosome assembly. UTP18/WDR50 is a member of the WD repeat family. The WD repeat is defined by four or more repeating units of a conserved core of approximately 40 amino acids ending with tryptophan-aspartic acid (WD). WD repeats may serve as sites of protein-protein interaction for adaptor proteins and facilitate multiprotein complex formation.

Alternate Names

CGI-48, U3 small nucleolar RNA-associated protein 18 homolog, UTP18, small subunit (SSU) processome component, homolog (yeast), WD repeat domain 50, WD repeat-containing protein 50, WDR50

Gene Symbol

UTP18

Additional UTP18 Products

Product Documents for UTP18 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for UTP18 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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