V-type proton ATPase subunit F Antibody (OTI1B8) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-74863

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI1B8

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all V-type proton ATPase subunit F Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human ATP6V1F (NP_004222) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

13.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for V-type proton ATPase subunit F Antibody (OTI1B8) - Azide and BSA Free

Western Blot: V-type proton ATPase subunit F Antibody (OTI1B8)Azide and BSA Free [NBP2-74863]

Western Blot: V-type proton ATPase subunit F Antibody (OTI1B8)Azide and BSA Free [NBP2-74863]

Western Blot: V-type proton ATPase subunit F Antibody (OTI1B8) - Azide and BSA Free [NBP2-74863] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY V-type proton ATPase subunit F (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted w
Immunocytochemistry/ Immunofluorescence: V-type proton ATPase subunit F Antibody (OTI1B8) - Azide and BSA Free [NBP2-74863]

Immunocytochemistry/ Immunofluorescence: V-type proton ATPase subunit F Antibody (OTI1B8) - Azide and BSA Free [NBP2-74863]

Immunocytochemistry/Immunofluorescence: V-type proton ATPase subunit F Antibody (OTI1B8) - Azide and BSA Free [NBP2-74863] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY V-type proton ATPase subunit F.
Immunohistochemistry: V-type proton ATPase subunit F Antibody (OTI1B8) - Azide and BSA Free [NBP2-74863]

Immunohistochemistry: V-type proton ATPase subunit F Antibody (OTI1B8) - Azide and BSA Free [NBP2-74863]

Immunohistochemistry: V-type proton ATPase subunit F Antibody (OTI1B8) - Azide and BSA Free [NBP2-74863] - Staining of paraffin-embedded Human prostate tissue using anti-V-type proton ATPase subunit F mouse monoclonal antibody.
View 8 more images

Applications for V-type proton ATPase subunit F Antibody (OTI1B8) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: V-type proton ATPase subunit F

V-type proton ATPase subunit F encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c", and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is the V1 domain F subunit protein. [provided by RefSeq]

Alternate Names

ATP6S14adenosinetriphosphatase 14k chain, ATPase, H+ transporting, lysosomal 14kDa, V1 subunit F, H(+)-transporting two-sector ATPase, 14kD subunit, MGC117321, MGC126037, MGC126038, vacuolar ATP synthase subunit F, vacuolar proton pump F subunit, Vacuolar proton pump subunit F, VATFATPase, vacuolar, 14 kD, V-ATPase 14 kDa subunit, V-ATPase F subunit, V-ATPase subunit F, Vma7, V-type proton ATPase subunit F

Gene Symbol

ATP6V1F

Additional V-type proton ATPase subunit F Products

Product Documents for V-type proton ATPase subunit F Antibody (OTI1B8) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for V-type proton ATPase subunit F Antibody (OTI1B8) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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