V5 Epitope Tag Antibody (1071)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-43626
Key Product Details
Species Reactivity
Epitope Tag
Applications
Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 1071
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
The immunogen used to generate this antibody corresponds to V5 Epitope Tag
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for V5 Epitope Tag Antibody (1071)
Western Blot: V5 Epitope Tag Antibody (1071) [NBP2-43626]
Western Blot: V5 Epitope Tag Antibody (1071) [NBP2-43626] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with V5 tag antibody [GT1071] diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.Immunocytochemistry/ Immunofluorescence: V5 Epitope Tag Antibody (1071) [NBP2-43626]
Immunocytochemistry/Immunofluorescence: V5 Epitope Tag Antibody (1071) [NBP2-43626] - V5-tag tagged protein transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: V5 tag stained by V5 tag antibody [1071] diluted at 1:500.Blue: Hoechst 33342 staining.Immunoprecipitation: V5 Epitope Tag Antibody (1071) [NBP2-43626]
Immunoprecipitation: V5 Epitope Tag Antibody (1071) [NBP2-43626] - Analysis of 400 ug cell lysate/extract of V5-fused gene transfected 293T cells A. 30 ug lysate/extract of transfected 293T cell B. Control with 2 ug of preimmune mouse IgG C. Immunoprecipitation of V5-fused protein by 2 ug of V5 tag antibody [1071] 12% SDS-PAGE The immunoprecipitated V5-fused protein was detected by V5 tag antibody diluted at 1:1000. EasyBlot anti-mouse IgG was used as a secondary reagent.Applications for V5 Epitope Tag Antibody (1071)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:2000
Immunoprecipitation
1:100-1:500
Western Blot
1:500-1:3000
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: V5 Epitope Tag
Alternate Names
GKPIPNPLLGLDST Epitope Tag, Parainfluenza virus 5 V/P, Paramyxovirus SV5
Additional V5 Epitope Tag Products
Product Documents for V5 Epitope Tag Antibody (1071)
Product Specific Notices for V5 Epitope Tag Antibody (1071)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...