Paramyxovirus SV5 Pk Antibody (SV5-Pk1)
Novus Biologicals, part of Bio-Techne | Catalog # NB100-62264
![Western Blot: Paramyxovirus SV5 Pk Antibody (SV5-Pk1)BSA Free [NB100-62264]](https://resources.bio-techne.com/images/products/Paramyxovirus-SV5-Pk-Antibody-SV5-Pk1-Western-Blot-NB100-62264-img0004.jpg "Western Blot: Paramyxovirus SV5 Pk Antibody (SV5-Pk1)BSA Free [NB100-62264]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Viral
Cited:
Mouse
Applications
Validated:
ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunoprecipitation, Radioimmunoassay, Sandwich ELISA, Western Blot
Cited:
IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Frozen, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # SV5-Pk1
Concentration
1.0 mg/ml
Product Specifications
Immunogen
Paramyxovirus Simian-Virus 5 (SV5)
Reactivity Notes
Epitope tag. Non-species specific. Mouse reactivity reported in scientific literature (PMID: 27681617).
Specificity
This product recognises a small epitope, termed Pk, present on the P/V proteins of the paramyxovirus, SV5. NB100-62264 has been used to detect recombinant proteins, some of which include transmembrane and secreted proteins, which have been tagged with this epitope. Usually, a 14 amino acid tag has been added to the recombinant proteins, although a smaller epitope of 9 amino acids (that as a peptide inhibit the binding of the monoclonal antibody to its native protein) has also been successfully used. The 14 amino acid epitope is; gly lys pro (ile pro asn pro leu leu gly leu asp) ser thr. (9 amino acid epitope is in parenthesis)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Paramyxovirus SV5 Pk Antibody (SV5-Pk1)
Western Blot: Paramyxovirus SV5 Pk Antibody (SV5-Pk1)BSA Free [NB100-62264]
Western Blot: Paramyxovirus SV5 Pk Antibody (SV5-Pk1) [NB100-62264] - Analysis using the Biotin conjugate of NB100-62264. Detection of V5 tagged protein with Mouse anti V5-tag.
Sandwich ELISA: Paramyxovirus SV5 Pk Antibody (SV5-Pk1) [NB100-62264] - V5 tagged protein detected with Mouse anti V5-Tag.
Applications for Paramyxovirus SV5 Pk Antibody (SV5-Pk1)
Application
Recommended Usage
ELISA
1:1000-1:5000
Flow Cytometry
1:10-1:1000
Immunocytochemistry/ Immunofluorescence
1:10-1:500
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Frozen
1:10-1:500
Immunoprecipitation
1:10-1:500RadioImmunoassay 1:100-1:2000
Radioimmunoassay
1:100-1:2000
Western Blot
1:1000-1:5000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Paramyxovirus SV5 Pk
Alternate Names
Simian virus 5; SPV5gp2; V protein
UniProt
Additional Paramyxovirus SV5 Pk Products
Product Documents for Paramyxovirus SV5 Pk Antibody (SV5-Pk1)
Product Specific Notices for Paramyxovirus SV5 Pk Antibody (SV5-Pk1)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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