Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Cited:

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # S6-38

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all VAChT/SLC18A3 Antibodies »

Product Specifications

Immunogen

Synthetic peptide amino acids 521-532 of human VAChT

Localization

Membrane

Specificity

Detects 56kDa.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for VAChT/SLC18A3 Antibody (S6-38)

Western Blot: VAChT/SLC18A3 Antibody (S6-38) [NBP2-59378]

Western Blot: VAChT/SLC18A3 Antibody (S6-38) [NBP2-59378]

Western Blot: VAChT/SLC18A3 Antibody (S6-38) [NBP2-59378] - Western Blot analysis of Rat brain membrane lysate showing detection of VAChT/SLC18A3 protein using Mouse Anti-VAChT/SLC18A3 Monoclonal Antibody, Clone S6-38 (NBP2-59378). Primary Antibody: Mouse Anti-VAChT/SLC18A3 Monoclonal Antibody (NBP2-59378) at 1:1000.
Immunocytochemistry/ Immunofluorescence: VAChT/SLC18A3 Antibody (S6-38) [NBP2-59378]

Immunocytochemistry/ Immunofluorescence: VAChT/SLC18A3 Antibody (S6-38) [NBP2-59378]

Immunocytochemistry/Immunofluorescence: VAChT/SLC18A3 Antibody (S6-38) [NBP2-59378] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-VAChT/SLC18A3 Monoclonal Antibody, Clone S6-38 (NBP2-59378). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-VAChT/SLC18A3 Monoclonal Antibody (NBP2-59378) at 1:200 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) VAChT/SLC18A3 Antibody (D) Composite.
Immunocytochemistry/ Immunofluorescence: VAChT/SLC18A3 Antibody (S6-38) [NBP2-59378]

Immunocytochemistry/ Immunofluorescence: VAChT/SLC18A3 Antibody (S6-38) [NBP2-59378]

Immunocytochemistry/Immunofluorescence: VAChT/SLC18A3 Antibody (S6-38) [NBP2-59378] - Analysis using Mouse Anti-VAChT Monoclonal Antibody, Clone S6-38. Tissue: SK-N-BE Cells (Human Neuroblastoma cells). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-VAChT Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Membrane. Magnification: 60X.

Applications for VAChT/SLC18A3 Antibody (S6-38)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:200

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 50% Glycerol

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Background: VAChT/SLC18A3

Vesicular Acetylcholine Transporter (VAChT), (approx. 70kD protein), belongs to the family of vesicular monoamine transporters(VMATs), which include VMAT1 and VMAT2 and the C.elegans putative ACh transporter unc-17. Members of this family function to concentrate neurotransmitters into synaptic vesicles through exchange of protons for neurotransmitters. VAChT is a functional transporter for the neurotransmitter acetylcholine(ACh). ACh is synthesized in the cytoplasm by choline acetyl transferase (ChAT) and transported by VAChT into synaptic vesicles where it is stored until released. After release from presynaptic nerve terminals ACh is hydrolyzed by extracellular ACh-esterases(AChE) to choline and acetate. VAChT mRNA is expressed in all known major cholinergic neurons in the central and peripheral nervous system. VAChT is abundantly expressed in the CNS and is mainly localized in small synaptic vesicles in cholinergic nerve terminals. VAChT provides a specific marker for cholinergic neurons for the study of cholinergic transmission in experimental models, in Alzheimer's disease and other nervous system disorders.

Long Name

Vesicular Acetylcholine Transporter/Solute Carrier Family 18 Member 3

Alternate Names

SLC18A3

Gene Symbol

SLC18A3

Additional VAChT/SLC18A3 Products

Product Documents for VAChT/SLC18A3 Antibody (S6-38)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for VAChT/SLC18A3 Antibody (S6-38)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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