VAM1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-1366

Novus Biologicals, part of Bio-Techne
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NB100-1366
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Canine (100%), Mouse (100%), Porcine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all VAM1 Antibodies »

Product Specifications

Immunogen

Peptide with sequence QQVLENLTELPSS-C corresponding to N-Terminus according to NP_057531.2.

Localization

Plasma membrane

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for VAM1 Antibody

Western Blot: VAM1 Antibody [NB100-1366]

Western Blot: VAM1 Antibody [NB100-1366]

Western Blot: VAM1 Antibody [NB100-1366] - Staining of HEK293 cell lysate (RIPA buffer, 35 ug total protein per lane). Antibody at 0.3 ug/mL. Detected by Western blot using chemiluminescence.
Immunohistochemistry-Paraffin: VAM1 Antibody [NB100-1366]

Immunohistochemistry-Paraffin: VAM1 Antibody [NB100-1366]

Immunohistochemistry-Paraffin: VAM1 Antibody [NB100-1366] - Staining of paraffin embedded Human Liver. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for VAM1 Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

5 ug/mL

Peptide ELISA

Detection limit 1:128000

Western Blot

0.3 - 1 ug/mL
Application Notes
WB: Approx. 60 kDa band observed in HEK293 lysates (calculated MW of 61.1 kDa band according to NP_057531.2). An additional band of unknown identity was also consistently observed at 28 kDa. This band was successfully blocked by incubation with the immunizing peptide.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: VAM1

Members of the peripheral membrane-associated guanylate kinase (MAGUK) family function in tumor suppression and receptor clustering by forming multiprotein complexes containing distinct sets of transmembrane, cytoskeletal, and cytoplasmic signaling proteins. All MAGUKs contain a PDZ-SH3-GUK core and are divided into 4 subfamilies, DLG-like, ZO1-like, p55-like and LIN2-like, based on their size and the presence of additional domains. MPP6 is a member of the p55-like MAGUK subfamily.

Alternate Names

MAGUK protein p55T, membrane protein, palmitoylated 6 (MAGUK p55 subfamily member 6), p55T, protein associated with Lin7 2, VAM-1PALS2VAM1MAGUK p55 subfamily member 6, Veli-associated MAGUK 1

Entrez Gene IDs

51678 (Human); 56524 (Mouse)

Gene Symbol

MPP6

UniProt

NP_057531. (Human)

Additional VAM1 Products

Product Documents for VAM1 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for VAM1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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