VAMP-1 Antibody (5A4)

Novus Biologicals, part of Bio-Techne | Catalog # H00006843-M02

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Product Datasheet / COA / SDS

Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Lambda Clone # 5A4

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

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Product Specifications

Immunogen

VAMP1 (NP_055046, 28 a.a. ~ 96 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MTSNRRLQQTQAQVEEVVDIIRVNVDKVLERDQKLSELDDRADALQAGASQFESSAAKLKRKYWWKNCK

Specificity

VAMP1 (5A4)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Lambda

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for VAMP-1 Antibody (5A4)

Western Blot: VAMP-1 Antibody (5A4) [H00006843-M02]

Western Blot: VAMP-1 Antibody (5A4) [H00006843-M02] - VAMP1 monoclonal antibody (M02), clone 5A4 Analysis of VAMP1 expression in HepG2.

Immunocytochemistry/ Immunofluorescence: VAMP-1 Antibody (5A4) [H00006843-M02]

Immunocytochemistry/Immunofluorescence: VAMP-1 Antibody (5A4) [H00006843-M02] - Analysis of monoclonal antibody to VAMP1 on HepG2 cell. Antibody concentration 10 ug/ml.

Immunohistochemistry-Paraffin: VAMP-1 Antibody (5A4) [H00006843-M02]

Immunohistochemistry-Paraffin: VAMP-1 Antibody (5A4) [H00006843-M02] - Analysis of monoclonal antibody to VAMP1 on formalin-fixed paraffin-embedded human pancreas. Antibody concentration 1.5 ug/ml.

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Applications for VAMP-1 Antibody (5A4)

Application

Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Application Notes

Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF, IHC-P and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: VAMP-1

Synaptobrevins/VAMPs, syntaxins, and the 25-kD synaptosomal-associated protein SNAP25 are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane. VAMP1 is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family. Multiple alternative splice variants that encode proteins with alternative carboxy ends have been described, but the full-length nature of some variants has not been defined.

Long Name

Vesicle-Associated Membrane Protein 1

Alternate Names

SYB1, Synaptobrevin-1, VAMP1

Entrez Gene IDs

6843 (Human)

Gene Symbol

VAMP1

OMIM

185880 (Human)

UniProt

NP_055046 (Human)

Additional VAMP-1 Products

Product Documents for VAMP-1 Antibody (5A4)

Product Datasheets

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COA

SDS

Product Specific Notices for VAMP-1 Antibody (5A4)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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