VAMP-1 Antibody (5F3)
Novus Biologicals, part of Bio-Techne | Catalog # H00006843-M01
Key Product Details
Species Reactivity
Human
Applications
ELISA, Sandwich ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Lambda Clone # 5F3
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
VAMP1 (NP_055046, 28 a.a. ~ 96 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MTSNRRLQQTQAQVEEVVDIIRVNVDKVLERDQKLSELDDRADALQAGASQFESSAAKLKRKYWWKNCK
Specificity
VAMP1 - vesicle-associated membrane protein 1 (synaptobrevin 1) (5F3)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Lambda
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for VAMP-1 Antibody (5F3)
![Western Blot: VAMP-1 Antibody (5F3) [H00006843-M01]](https://resources.bio-techne.com/images/products/VAMP-1-Antibody-5F3-Western-Blot-H00006843-M01-img0004.jpg "Western Blot: VAMP-1 Antibody (5F3) [H00006843-M01]")
Western Blot: VAMP-1 Antibody (5F3) [H00006843-M01]
Western Blot: VAMP-1 Antibody (5F3) [H00006843-M01] - VAMP1 monoclonal antibody (M01), clone 5F3. Analysis of VAMP1 expression in HepG2.
Sandwich ELISA: VAMP-1 Antibody (5F3) [H00006843-M01] - Detection limit for recombinant GST tagged VAMP1 is approximately 0.1ng/ml as a capture antibody.
Applications for VAMP-1 Antibody (5F3)
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Sandwich ELISA
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: VAMP-1
Long Name
Vesicle-Associated Membrane Protein 1
Alternate Names
SYB1, Synaptobrevin-1, VAMP1
Entrez Gene IDs
6843 (Human)
Gene Symbol
VAMP1
UniProt
Additional VAMP-1 Products
Product Documents for VAMP-1 Antibody (5F3)
Product Specific Notices for VAMP-1 Antibody (5F3)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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