VAMP-1/VAMP-2 Antibody (SP10)

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-19332

Novus Biologicals, part of Bio-Techne
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NBP1-19332
NBP1-19332-0.025ml
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Porcine, Bovine, Hamster, Xenopus

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgM Clone # SP10

Concentration

1.0 mg/ml

View all VAMP-1/VAMP-2 Antibodies »

Product Specifications

Immunogen

Synaptic vesicle-containing fractions immunoprecipitated from human brain homogenates using anti-human Synaptophysin monoclonal antibodies.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Specificity

VAMP2 (SP10)

Marker

Synaptic Vesicles Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgM

Scientific Data Images for VAMP-1/VAMP-2 Antibody (SP10)

Western Blot: VAMP-1/VAMP-2 Antibody (SP10) [NBP1-19332]

Western Blot: VAMP-1/VAMP-2 Antibody (SP10) [NBP1-19332]

Western Blot: VAMP-2 Antibody (SP10) [NBP1-19332] - Analysis of mouse brain tissue extract.
Immunohistochemistry-Paraffin: VAMP-1/VAMP-2 Antibody (SP10) [NBP1-19332]

Immunohistochemistry-Paraffin: VAMP-1/VAMP-2 Antibody (SP10) [NBP1-19332]

Immunohistochemistry-Paraffin: VAMP-2 Antibody (SP10) [NBP1-19332] - Analysis of mouse brain tissue extract
Immunohistochemistry: VAMP-1/VAMP-2 Antibody (SP10) [NBP1-19332]

Immunohistochemistry: VAMP-1/VAMP-2 Antibody (SP10) [NBP1-19332]

Immunohistochemistry: VAMP-2 Antibody (SP10) [NBP1-19332] - Analysis of Xenopus embryonic brain, stained with VAMP2, mAb (SP10).

Applications for VAMP-1/VAMP-2 Antibody (SP10)

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1:4000

Formulation, Preparation, and Storage

Purification

Ammonium sulfate precipitation

Formulation

PBS (pH 7.2), 0.1mM PMSF and 50% Glycerol

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: VAMP-1/VAMP-2

VAMP2 (Synaptobrevin 2) is a small integral membrane protein found specifically in synaptic vesicles in neurons. Synaptobrevins/VAMPs, syntaxins, and the 25 kD synaptosomal associated protein SNAP25 are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane. VAMP2 is one of a family of integral membrane proteins of 11-13 kDa with the N terminal region exposed to the cytoplasm and a C terminal transmembrane domain. Two isoforms identified in the mammalian CNS Synaptobrevin 1/VAMP 1 and Synaptobrevin 2/VAMP 2 differ in their distribution within different brain regions. Synaptobrevin/VAMP is highly conserved between vertebrates and invertebrates. It is a major constituent of synaptic vesicles and peptidergic secretory granules in all neurons examined so far. In addition, it is present on secretory granules of neuroendocrine cells. Low levels of synaptobrevin 2/VAMP 2 are present in many other tissues where the protein resides on specialized microvesicles. In non-neuronal cells, the third isoform, Cellubrevin/VAMP 3, is present where it is localized to an endosomal membrane pool. Synaptobrevin/VAMP is an essential component of the exocytotic fusion machine, related to a larger protein family referred to as v SNAREs. It is the sole target for tetanus and several of the botulinal neurotoxins which cleave the protein at single sites in the C terminal portion of the molecule.

Long Name

Vesicle-Associated Membrane Protein 1/2

Alternate Names

SPAX1, SYB1, VAMP1, VAMP-1, vesicle associated membrane protein 1

Entrez Gene IDs

6844 (Human); 22318 (Mouse); 24803 (Rat)

Gene Symbol

VAMP2

Additional VAMP-1/VAMP-2 Products

Product Documents for VAMP-1/VAMP-2 Antibody (SP10)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for VAMP-1/VAMP-2 Antibody (SP10)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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