Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Microarray, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # N15/39

Concentration

1 mg/ml

View all Vanilloid R-like 3/TRPV3 Antibodies »

Product Summary for Vanilloid R-like 3/TRPV3 Antibody (N15/39)

Immunogen

Synthetic peptide amino acids 458-474 (cytoplasmic C-terminus) of rat TrpV3

Localization

Membrane

Specificity

Detects approx 70kDa.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

70 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Vanilloid R-like 3/TRPV3 Antibody (N15/39)

Western Blot: Vanilloid R-like 3/TRPV3 Antibody (N15/39) [NBP2-12918]

Western Blot: Vanilloid R-like 3/TRPV3 Antibody (N15/39) [NBP2-12918]

Western Blot: Vanilloid R-like 3/TRPV3 Antibody (N15/39) [NBP2-12918] - Western Blot analysis of Rat brain membrane lysate showing detection of TrpV3 protein using Mouse Anti-TrpV3 Monoclonal Antibody, Clone S15-39 (NBP2-12918). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-TrpV3 Monoclonal Antibody (NBP2-12918) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.
Immunocytochemistry/ Immunofluorescence: Vanilloid R-like 3/TRPV3 Antibody (N15/39) [NBP2-12918]

Immunocytochemistry/ Immunofluorescence: Vanilloid R-like 3/TRPV3 Antibody (N15/39) [NBP2-12918]

Immunocytochemistry/Immunofluorescence: Vanilloid R-like 3/TRPV3 Antibody (N15/39) [NBP2-12918] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-TrpV3 Monoclonal Antibody, Clone S15-39 (NBP2-12918). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-TrpV3 Monoclonal Antibody (NBP2-12918) at 1:50 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) TrpV3 Antibody (D) Composite.
Immunohistochemistry: Vanilloid R-like 3/TRPV3 Antibody (N15/39) [NBP2-12918]

Immunohistochemistry: Vanilloid R-like 3/TRPV3 Antibody (N15/39) [NBP2-12918]

Immunohistochemistry: Vanilloid R-like 3/TRPV3 Antibody (N15/39) [NBP2-12918] - Immunohistochemistry analysis using Mouse Anti-TrpV3 Monoclonal Antibody, Clone S15-39 (NBP2-12918). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-TrpV3 Monoclonal Antibody (NBP2-12918) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Filaggrin-like staining in upper layers. Dull lower layer cell staining. Some stain seen in hypodermis.
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Applications for Vanilloid R-like 3/TRPV3 Antibody (N15/39)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:1000

Western Blot

1:1000
Application Notes
1 ug/ml of TrpV3 Antibody was sufficient for detection of TrpV3 in 10 ug of COS-1 cell lysate transiently transfected with TrpV3 by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary Antibody.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 50% Glycerol

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Vanilloid R-like 3/TRPV3

1 ug/mL was sufficient for detection of TrpV3 in 10ug of COS-1 cell lysate transiently transfected with TrpV3 by colorimetric immunoblot analysis using Goat anti-mouse IgG: HRP as the secondary antibody

Long Name

Vanilloid Receptor Like 3/Transient Receptor Potential Cation Channel 3

Alternate Names

TRPV3, VanilloidR like 3, VanilloidR-like3, VRL3

Gene Symbol

TRPV3

Additional Vanilloid R-like 3/TRPV3 Products

Product Documents for Vanilloid R-like 3/TRPV3 Antibody (N15/39)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Vanilloid R-like 3/TRPV3 Antibody (N15/39)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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