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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI10E10

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 2-227 of human VAPA (NP_919415) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

27.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for VAP-A Antibody (OTI10E10) - Azide and BSA Free

Western Blot: VAP-A Antibody (OTI10E10)Azide and BSA Free [NBP2-74825]

Western Blot: VAP-A Antibody (OTI10E10)Azide and BSA Free [NBP2-74825]

Western Blot: VAP-A Antibody (OTI10E10) - Azide and BSA Free [NBP2-74825] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VAP-A.
Immunohistochemistry: VAP-A Antibody (OTI10E10) - Azide and BSA Free [NBP2-74825]

Immunohistochemistry: VAP-A Antibody (OTI10E10) - Azide and BSA Free [NBP2-74825]

Immunohistochemistry: VAP-A Antibody (OTI10E10) - Azide and BSA Free [NBP2-74825] - Analysis of Human thyroid tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: VAP-A Antibody (OTI10E10) - Azide and BSA Free [NBP2-74825]

Immunohistochemistry: VAP-A Antibody (OTI10E10) - Azide and BSA Free [NBP2-74825]

Immunohistochemistry: VAP-A Antibody (OTI10E10) - Azide and BSA Free [NBP2-74825] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)

Applications for VAP-A Antibody (OTI10E10) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: VAP-A

VAPA is encoded by this gene is a type IV membrane protein. It is present in the plasma membrane and intracellular vesicles. It may also be associated with the cytoskeleton. This protein may function in vesicle trafficking, membrane fusion, protein complex assembly and cell motility. Alternative splicing occurs at this locus and two transcript variants encoding distinct isoforms have been identified. [provided by RefSeq]

Long Name

VAMP [Vesicle-associated Membrane Protein]-associated Protein A

Alternate Names

VAMP-A, VAP-33, VAPA

Gene Symbol

VAPA

Additional VAP-A Products

Product Documents for VAP-A Antibody (OTI10E10) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for VAP-A Antibody (OTI10E10) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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