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VAP-A Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-31237

Novus Biologicals, part of Bio-Techne
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NBP1-31237

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse

Predicted:

Bovine (100%), Chicken (100%), Chimpanzee (100%), Zebrafish (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human VAP-A. The exact sequence is proprietary.

Localization

Cell membrane, Single-pass type IV membrane protein, Endomembrane system, Single-pass type IV membrane protein |

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

28 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for VAP-A Antibody

Western Blot: VAP-A Antibody [NBP1-31237]

Western Blot: VAP-A Antibody [NBP1-31237]

Western Blot: VAP-A Antibody [NBP1-31237] - Rat tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with VAPA antibody [N1N3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: VAP-A Antibody [NBP1-31237]

Immunocytochemistry/ Immunofluorescence: VAP-A Antibody [NBP1-31237]

Immunocytochemistry/Immunofluorescence: VAP-A Antibody [NBP1-31237] - WDR44 tubules are in close contact with the ER via binding to VAPA/B. Confocal images of transfected HeLa cells showing colocalization of GRAF2-RFP tubules with GFP-VAPA. Image collected and cropped by CiteAb from the following publication (//rupress.org/jcb/article/doi/10.1083/jcb.201811014/151714/GRAF2-WDR44-and-MICAL1-mediate-Rab81011dependent) licensed under a CC-BY license.
Immunohistochemistry-Paraffin: VAP-A Antibody [NBP1-31237]

Immunohistochemistry-Paraffin: VAP-A Antibody [NBP1-31237]

Immunohistochemistry-Paraffin: VAP-A Antibody [NBP1-31237] - Human breast cancer, using VAPA antibody at 1:500 dilution.

Applications for VAP-A Antibody

Application
Recommended Usage

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris-Glycine (pH7), 10% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: VAP-A

The protein encoded by this gene is a type IV membrane protein. It is present in the plasma membrane and intracellular vesicles. It may also be associated with the cytoskeleton. This protein may function in vesicle trafficking, membrane fusion, protein complex assembly and cell motility. Alternative splicing occurs at this locus and two transcript variants encoding distinct isoforms have been identified. [provided by RefSeq]

Long Name

VAMP [Vesicle-associated Membrane Protein]-associated Protein A

Alternate Names

VAMP-A, VAP-33, VAPA

Gene Symbol

VAPA

Additional VAP-A Products

Product Documents for VAP-A Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for VAP-A Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.