VAP-A Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-31237
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human, Mouse
Predicted:
Bovine (100%), Chicken (100%), Chimpanzee (100%), Zebrafish (100%). Backed by our 100% Guarantee.
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human VAP-A. The exact sequence is proprietary.
Localization
Cell membrane, Single-pass type IV membrane protein, Endomembrane system, Single-pass type IV membrane protein |
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
28 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for VAP-A Antibody
Western Blot: VAP-A Antibody [NBP1-31237]
Western Blot: VAP-A Antibody [NBP1-31237] - Rat tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with VAPA antibody [N1N3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.Immunocytochemistry/ Immunofluorescence: VAP-A Antibody [NBP1-31237]
Immunocytochemistry/Immunofluorescence: VAP-A Antibody [NBP1-31237] - WDR44 tubules are in close contact with the ER via binding to VAPA/B. Confocal images of transfected HeLa cells showing colocalization of GRAF2-RFP tubules with GFP-VAPA. Image collected and cropped by CiteAb from the following publication (//rupress.org/jcb/article/doi/10.1083/jcb.201811014/151714/GRAF2-WDR44-and-MICAL1-mediate-Rab81011dependent) licensed under a CC-BY license.Immunohistochemistry-Paraffin: VAP-A Antibody [NBP1-31237]
Immunohistochemistry-Paraffin: VAP-A Antibody [NBP1-31237] - Human breast cancer, using VAPA antibody at 1:500 dilution.Applications for VAP-A Antibody
Application
Recommended Usage
Immunohistochemistry
1:100-1:1000
Immunohistochemistry-Paraffin
1:100-1:1000
Western Blot
1:500-1:3000
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
0.1M Tris-Glycine (pH7), 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: VAP-A
Long Name
VAMP [Vesicle-associated Membrane Protein]-associated Protein A
Alternate Names
VAMP-A, VAP-33, VAPA
Gene Symbol
VAPA
Additional VAP-A Products
Product Documents for VAP-A Antibody
Product Specific Notices for VAP-A Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.