VAP-A Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-83132

Novus Biologicals, part of Bio-Techne
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NBP1-83132
NBP1-83132-25ul
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all VAP-A Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: NDKLGITPPGNAPTVTSMSSINNTVATPASYHTKDDPRGLSVLKQEKQKNDMEPSKAVPLNASKQDGPMPKPHSVSLNDTETRKLMEECKRLQGEMMKLSEENRHLRDEGLRLRKVAHSDKPGSTSTASF

Reactivity Notes

Rat (84%).

Localization

Endoplasmic Reticulum

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

28 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for VAP-A Antibody

Western Blot: VAP-A Antibody [NBP1-83132]

Western Blot: VAP-A Antibody [NBP1-83132]

Western Blot: VAP-A Antibody [NBP1-83132] - Analysis in U-138MG cells transfected with control siRNA, target specific siRNA probe #1 and #2,. Remaining relative intensity is presented. Loading control: Anti-PPIB.
Immunocytochemistry/ Immunofluorescence: VAP-A Antibody [NBP1-83132]

Immunocytochemistry/ Immunofluorescence: VAP-A Antibody [NBP1-83132]

Immunocytochemistry/Immunofluorescence: VAP-A Antibody [NBP1-83132] - Staining of human cell line U-2 OS shows localization to endoplasmic reticulum. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: VAP-A Antibody [NBP1-83132]

Immunohistochemistry-Paraffin: VAP-A Antibody [NBP1-83132]

Immunohistochemistry-Paraffin: VAP-A Antibody [NBP1-83132] - Staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.
View 1 more images

Applications for VAP-A Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Reviewed Applications

Read 1 review rated 5 using NBP1-83132 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: VAP-A

VAPA is encoded by this gene is a type IV membrane protein. It is present in the plasma membrane and intracellular vesicles. It may also be associated with the cytoskeleton. This protein may function in vesicle trafficking, membrane fusion, protein complex assembly and cell motility. Alternative splicing occurs at this locus and two transcript variants encoding distinct isoforms have been identified. [provided by RefSeq]

Long Name

VAMP [Vesicle-associated Membrane Protein]-associated Protein A

Alternate Names

VAMP-A, VAP-33, VAPA

Entrez Gene IDs

9218 (Human)

Gene Symbol

VAPA

UniProt

Q9P0L0 (Human)

Additional VAP-A Products

Product Documents for VAP-A Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for VAP-A Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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