VAP-A Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-32249
Conjugate
Catalog #
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human, Mouse
Predicted:
Primate (100%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
1.0 mg/ml
Product Specifications
Immunogen
The immunogen this antibody was made to, maps to a region between residue 125 to 175 of human 33 kDa VAMP-Associated Protein using the numbering given in entry NP_919415.2 (GeneID 9218).
Reactivity Notes
Orangutan (100%).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for VAP-A Antibody
Western Blot: VAP-A Antibody [NBP2-32249]
Western Blot: VAP-A Antibody [NBP2-32249] - Samples: Whole cell lysate (50 ug) prepared using NETN or RIPA buffer from HeLa, 293T, Jurkat, and mouse TCMK-1 cells. Antibodies: Affinity purified rabbit anti-VAPA antibody NBP2-32249 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.Immunohistochemistry: VAP-A Antibody [NBP2-32249]
Immunohistochemistry: VAP-A Antibody [NBP2-32249] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- VAP-33 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).Immunoprecipitation: VAP-A Antibody [NBP2-32249]
Immunoprecipitation: VAP-A Antibody [NBP2-32249] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) prepared using NETN buffer from 293T cells. Antibodies: Affinity purified rabbit anti-VAPA antibody NBP2-32249 used for IP at 6 ug per reaction. VAPA was also immunoprecipitated by rabbit anti-VAPA antibody BL16444. For blotting immunoprecipitated VAPA, NBP2-32249 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.Applications for VAP-A Antibody
Application
Recommended Usage
Immunohistochemistry
1:500 - 1:2000
Immunohistochemistry-Paraffin
1:500 - 1:2000
Immunoprecipitation
2 - 10 ug/mg lysate
Western Blot
1:1000 - 1:5000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: VAP-A
Long Name
VAMP [Vesicle-associated Membrane Protein]-associated Protein A
Alternate Names
VAMP-A, VAP-33, VAPA
Gene Symbol
VAPA
UniProt
Additional VAP-A Products
Product Documents for VAP-A Antibody
Product Specific Notices for VAP-A Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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