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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse

Predicted:

Primate (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

Product Specifications

Immunogen

The immunogen this antibody was made to, maps to a region between residue 125 to 175 of human 33 kDa VAMP-Associated Protein using the numbering given in entry NP_919415.2 (GeneID 9218).

Reactivity Notes

Orangutan (100%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for VAP-A Antibody

Western Blot: VAP-A Antibody [NBP2-32249]

Western Blot: VAP-A Antibody [NBP2-32249]

Western Blot: VAP-A Antibody [NBP2-32249] - Samples: Whole cell lysate (50 ug) prepared using NETN or RIPA buffer from HeLa, 293T, Jurkat, and mouse TCMK-1 cells. Antibodies: Affinity purified rabbit anti-VAPA antibody NBP2-32249 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunohistochemistry: VAP-A Antibody [NBP2-32249]

Immunohistochemistry: VAP-A Antibody [NBP2-32249]

Immunohistochemistry: VAP-A Antibody [NBP2-32249] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- VAP-33 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).
Immunoprecipitation: VAP-A Antibody [NBP2-32249]

Immunoprecipitation: VAP-A Antibody [NBP2-32249]

Immunoprecipitation: VAP-A Antibody [NBP2-32249] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) prepared using NETN buffer from 293T cells. Antibodies: Affinity purified rabbit anti-VAPA antibody NBP2-32249 used for IP at 6 ug per reaction. VAPA was also immunoprecipitated by rabbit anti-VAPA antibody BL16444. For blotting immunoprecipitated VAPA, NBP2-32249 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Applications for VAP-A Antibody

Application
Recommended Usage

Immunohistochemistry

1:500 - 1:2000

Immunohistochemistry-Paraffin

1:500 - 1:2000

Immunoprecipitation

2 - 10 ug/mg lysate

Western Blot

1:1000 - 1:5000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: VAP-A

VAPA is encoded by this gene is a type IV membrane protein. It is present in the plasma membrane and intracellular vesicles. It may also be associated with the cytoskeleton. This protein may function in vesicle trafficking, membrane fusion, protein complex assembly and cell motility. Alternative splicing occurs at this locus and two transcript variants encoding distinct isoforms have been identified. [provided by RefSeq]

Long Name

VAMP [Vesicle-associated Membrane Protein]-associated Protein A

Alternate Names

VAMP-A, VAP-33, VAPA

Gene Symbol

VAPA

Additional VAP-A Products

Product Documents for VAP-A Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for VAP-A Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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