VARS Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-20843
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the N-terminus region of human VARS. The exact sequence is proprietary.
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (84%), Rat (86%).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
140 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for VARS Antibody
![Western Blot: VARS Antibody [NBP2-20843]](https://resources.bio-techne.com/images/products/VARS-Antibody-Western-Blot-NBP2-20843-img0001.jpg "Western Blot: VARS Antibody [NBP2-20843]")
Western Blot: VARS Antibody [NBP2-20843]
Western Blot: VARS Antibody [NBP2-20843] - Sample (30 ug of whole cell lysate) A: Hep G2 B: HCT116 5% SDS PAGE gel, diluted at 1:10000.![Immunocytochemistry/ Immunofluorescence: VARS Antibody [NBP2-20843]](https://resources.bio-techne.com/images/products/VARS-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-20843-img0003.jpg "Immunocytochemistry/ Immunofluorescence: VARS Antibody [NBP2-20843]")
Immunocytochemistry/ Immunofluorescence: VARS Antibody [NBP2-20843]
Immunocytochemistry/Immunofluorescence: VARS Antibody [NBP2-20843] - Analysis of methanol-fixed HeLa, using antibody at 1:200 dilution.
Immunohistochemistry: VARS Antibody [NBP2-20843] -
Immunohistochemistry: VARS Antibody [NBP2-20843] - VARS antibody [N1N2], N-term detects VARS protein at cytoplasm on human colon carcinoma by immunohistochemical analysis.Sample: Paraffin-embedded colon carcinoma.
VARS antibody [N1N2], N-term (NBP2-20843) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Applications for VARS Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Immunohistochemistry
1:100-1:1000
Immunohistochemistry-Paraffin
1:100-1:1000
Western Blot
1:5000-1:20000
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: VARS
Alternate Names
EC 6.1.1, EC 6.1.1.9, G7AVARS1, Protein G7a, valine tRNA ligase 1, cytoplasmic, Valine--tRNA ligase, ValRS, valyl-tRNA synthetase, valyl-tRNA synthetase 2, VARS2valRS
Gene Symbol
VARS1
UniProt
Additional VARS Products
Product Documents for VARS Antibody
Product Specific Notices for VARS Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.