VASA Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-20844
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Fish
Predicted:
Bovine (94%), Porcine (98%), Primate (100%), Rat (90%), Rhesus Macaque (99%). Backed by our 100% Guarantee.
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry Whole-Mount, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human VASA. The exact sequence is proprietary.
Reactivity Notes
Chimpanzee (100%), Siberian sturgeon (100%).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
79 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for VASA Antibody
Western Blot: VASA Antibody [NBP2-20844]
Western Blot: VASA Antibody [NBP2-20844] - Sample (30 ug of whole cell lysate) A: U87-MG 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.Immunohistochemistry-Paraffin: VASA Antibody [NBP2-20844]
Immunohistochemistry-Paraffin: VASA Antibody [NBP2-20844] - Paraffin-embedded mouse testis. DDX4 antibody [C1C3] diluted at 1:500.Western Blot: VASA Antibody [NBP2-20844] -
Western Blot: VASA Antibody [NBP2-20844] - Mouse tissue extract (30 | 50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with VASA antibody [C1C3] (NBP2-20844) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Applications for VASA Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Immunohistochemistry
1:100-1:1000
Immunohistochemistry Whole-Mount
1:10 - 1:500
Immunohistochemistry-Paraffin
1:100-1:1000
Western Blot
1:500-1:3000
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol
Preservative
0.025% Proclin 300
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: VASA
Long Name
DEAD [Asp-Glu-Ala-Asp] Box Polypeptide 41
Alternate Names
DDX4
Gene Symbol
DDX4
UniProt
Additional VASA Products
Product Documents for VASA Antibody
Product Specific Notices for VASA Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.