VASP Antibody (M277)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-23263
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human
Applications
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # M277
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for VASP Antibody (M277)
Immunogen
Clone (M277) was generated from a recombinant protein that includes amino acids from the C-terminal region of human VASP. [UniProt# P50552]
Specificity
This antibody detects 46 and 50 kDa proteins corresponding to the apparent molecular mass of VASP on SDS-PAGE immunoblots of human A431, HeLa, and HUVEC.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
46 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Stable for at least 1 year at -20°C.
Scientific Data Images for VASP Antibody (M277)
Immunocytochemistry/Immunofluorescence: VASP Antibody (M277) [NBP3-23263] -
Immunocytochemistry/Immunofluorescence: VASP Antibody (M277) [NBP3-23263] - Immunocytochemical labeling of VASP in aldehyde-fixed and NP-40-permeabilized A431 cells. The cells were labeled with mouse monoclonal VASP (C-terminal region) antibody, then the antibody was detected using appropriate secondary antibody conjugated to DyLight(R) 594.
Western Blot: VASP Antibody (M277) [NBP3-23263] -
Western Blot: VASP Antibody (M277) [NBP3-23263] - Western blot image of human A431 cells stimulated with calyculin A (100 nM) for 30 min. The blots were untreated (lanes 1 & 3) or treated with lambda phosphatase (lanes 2 & 4), then probed with mouse monoclonal VASP (C-term.) antibody (lanes 1 & 2) or rabbit polyclonal VASP (Thr-278) phospho-specific antibody (lanes 3 & 4).Applications for VASP Antibody (M277)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50
Western Blot
1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS, 1 mg/ml BSA and 50% glycerol
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C.
Background: VASP
Long Name
Vasodilator-stimulated Phosphoprotein
Alternate Names
vasodilator-stimulated phosphoprotein
Gene Symbol
VASP
Additional VASP Products
Product Documents for VASP Antibody (M277)
Product Specific Notices for VASP Antibody (M277)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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