VAT1 Antibody - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-93463

Novus Biologicals, part of Bio-Techne
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NBP2-93463-0.02ml
NBP2-93463-0.1ml
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Knockout Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all VAT1 Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 200-300 of human VAT1 (NP_006364.2). GVGMAAVQLCRTVENVTVFGTASASKHEALKENGVTHPIDYHTTDYVDEIKKISPKGVDIVMDPLGGSDTAKGYNLLKPMGKVVTYGMANLLTGPKRNLMA

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

42 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for VAT1 Antibody - Azide and BSA Free

Western Blot: VAT1 AntibodyAzide and BSA Free [NBP2-93463]

Western Blot: VAT1 AntibodyAzide and BSA Free [NBP2-93463]

Western Blot: VAT1 Antibody [NBP2-93463] - Analysis of extracts of various cell lines, using VAT1 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 5min.
Immunocytochemistry/ Immunofluorescence: VAT1 Antibody - Azide and BSA Free [NBP2-93463]

Immunocytochemistry/ Immunofluorescence: VAT1 Antibody - Azide and BSA Free [NBP2-93463]

Immunocytochemistry/Immunofluorescence: VAT1 Antibody [NBP2-93463] - Analysis of L929 cells using VAT1 at dilution of 1:100. Blue: DAPI for nuclear staining.
Knockout Validated: VAT1 Antibody - Azide and BSA Free [NBP2-93463]

Knockout Validated: VAT1 Antibody - Azide and BSA Free [NBP2-93463]

Knockout Validated: VAT1 Antibody [NBP2-93463] - Analysis of extracts from normal (control) and VAT1 knockout (KO) 293T cells, using VAT1 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.

Applications for VAT1 Antibody - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

Azide and BSA Free

Preservative

0.01% Thimerosal

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: VAT1

VAT1 - vesicle amine transport protein 1 homolog (T californica)

Alternate Names

FLJ20230, membrane protein of cholinergic synaptic vesicles, synaptic vesicle membrane protein VAT-1 homolog, VATI, vesicle amine transport protein 1 homolog (T. californica)

Gene Symbol

VAT1

Additional VAT1 Products

Product Documents for VAT1 Antibody - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for VAT1 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

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