Vav-1 Antibody (557)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-43553

Novus Biologicals, part of Bio-Techne
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NBP2-43553
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (96%), Porcine (96%), Rat (92%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 557

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Vav-1 Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Vav-1. The exact sequence is proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

98 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Vav-1 Antibody (557)

Western Blot: Vav-1 Antibody (557) [NBP2-43553]

Western Blot: Vav-1 Antibody (557) [NBP2-43553]

Western Blot: Vav-1 Antibody (557) [NBP2-43553] - Analysis of A. 30 ug Jurkat whole cell lysate/extract B. 30 ug NCI-H929 whole cell lysate/extract 7.5 % SDS-PAGE VAV1 antibody [557] dilution: 1:1000.
Immunocytochemistry/ Immunofluorescence: Vav-1 Antibody (557) [NBP2-43553]

Immunocytochemistry/ Immunofluorescence: Vav-1 Antibody (557) [NBP2-43553]

Immunocytochemistry/Immunofluorescence: Vav-1 Antibody (557) [NBP2-43553] - VAV1 antibody [GT557] detects VAV1 protein at cell membrane and cytoplasm by immunofluorescent analysis. Sample: Jurkat cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: VAV1 stained by VAV1 antibody [GT557] diluted at 1:500. Blue: Fluoroshield with DAPI. Scale bar= 10 um.
Immunohistochemistry-Paraffin: Vav-1 Antibody (557) [NBP2-43553]

Immunohistochemistry-Paraffin: Vav-1 Antibody (557) [NBP2-43553]

Immunohistochemistry-Paraffin: Vav-1 Antibody (557) [NBP2-43553] - VAV1 antibody [GT557] detects VAV1 protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse spleen. VAV1 stained by VAV1 antibody [GT557] diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Applications for Vav-1 Antibody (557)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Vav-1

The Vav family are Rho/Rac guanosine nucleotide exchange factors (GEFs), consisting of three members in mammalian cells (Vav, Vav2, Vav3) and one in nematodes (CelVav) (1). First discovered based on its transforming properties, Vav is expressed mainly in hematopoietic cells and a few non-hematopoietic tissues, such as the pancreas and tooth enamels (2). As a signaling transducer, Vav is involved in T-cell activated transduction of T-cell antigen receptor (TCR). T-cell stimulated and tyrosine phosphorylated Vav acts as a catalyst in the exchange of guanosine nucleotides on Rac-1, a GTP binding protein (3). Using a mouse model, Vav expression has been determined to play an essential role in the cyctosketetal, proliferative, and apoptotic pathways for developing lymphoid cells and its signal response (2).

Alternate Names

vav-T, Vav1

Gene Symbol

VAV1

Additional Vav-1 Products

Product Documents for Vav-1 Antibody (557)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Vav-1 Antibody (557)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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