VAV2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13026

Novus Biologicals, part of Bio-Techne
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NBP3-13026
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Chicken (91%), Rat (95%), Rhesus Macaque (100%). Backed by our 100% Guarantee.

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all VAV2 Antibodies »

Product Summary for VAV2 Antibody

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human VAV2. The exact sequence is proprietary.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Centrifuge briefly prior to opening.

Scientific Data Images for VAV2 Antibody

Western Blot: VAV2 Antibody [NBP3-13026]

Western Blot: VAV2 Antibody [NBP3-13026]

Western Blot: VAV2 Antibody [NBP3-13026] - Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with VAV2 antibody (NBP3-13026) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Western Blot: VAV2 Antibody [NBP3-13026]

Western Blot: VAV2 Antibody [NBP3-13026]

Western Blot: VAV2 Antibody [NBP3-13026] - VAV2 antibody detects VAV2 protein by western blot analysis. Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VAV2 antibody (NBP3-13026) diluted at a dilution of 1:1000.
Western Blot: VAV2 Antibody [NBP3-13026]

Western Blot: VAV2 Antibody [NBP3-13026]

Western Blot: VAV2 Antibody [NBP3-13026] - Immunoprecipitation of VAV2 protein from HepG2 whole cell extracts using 5 ug of VAV2 antibody (NBP3-13026). Western blot analysis was performed using VAV2 antibody (NBP3-13026) diluted at 1:500. EasyBlot anti-Rabbit IgG was used as a secondary reagent.

Applications for VAV2 Antibody

Application
Recommended Usage

Immunoprecipitation

1:100-1:705
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: VAV2

The Vav family are Rho/Rac guanosine nucleotide exchange factors (GEFs), consisting of three members in mammalian cells (Vav, Vav2, Vav3) and one in nematodes (CelVav) (1). Vav and Vav2 are members of the dbl family of guanine nucleotide exchange factors (GEF) for the rho/rac family of GTP binding proteins. Vav is expressed primarily in hematopoietic cells, while vav2 has a wider tissue distribution (2). Vav2 serves as a guanine nucleotide exchange factor for the Rho family of Ras-related GTPases. Unlike Vav1, whose expression is restricted to cells of hematopoietic origin, Vav2 is broadly expressed. Recently, Vav2 has been identified as a substrate for the epidermal growth factor (EGF) receptor. EGF receptor phosphorylates Vav2 on all three possible phosphorylation sites, Tyr-142, Tyr-159, and Tyr-172. results suggest that EGF regulates Vav2 activity basically through phosphatidylinositol 3-kinase activation, whereas tyrosine phosphorylation of Vav2 may rather be necessary for mediating protein-protein interactions (3)

Alternate Names

guanine nucleotide exchange factor VAV2, oncogene VAV2, Protein vav-2, vav 2 guanine nucleotide exchange factor, vav 2 oncogene, VAV-2

Gene Symbol

VAV2

Additional VAV2 Products

Product Documents for VAV2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for VAV2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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