VAV3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB300-817

Novus Biologicals, part of Bio-Techne
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NB300-817
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Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all VAV3 Antibodies »

Product Specifications

Immunogen

Peptide with sequence CSGEQGTLKLPEK corresponding to internal region according to NP_006104.4, NP_001073343.1.

Specificity

This antibody is expected to recognize both reported isoforms (NP_006104.4; NP_001073343.1).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for VAV3 Antibody

Immunocytochemistry/ Immunofluorescence: VAV3 Antibody [NB300-817]

Immunocytochemistry/ Immunofluorescence: VAV3 Antibody [NB300-817]

Immunocytochemistry/Immunofluorescence: VAV3 Antibody [NB300-817] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Immunohistochemistry-Paraffin: VAV3 Antibody [NB300-817]

Immunohistochemistry-Paraffin: VAV3 Antibody [NB300-817]

Immunohistochemistry-Paraffin: VAV3 Antibody [NB300-817] - Staining of paraffin embedded Human Spleen. Antibody at 7.5 ug/mL. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
Flow Cytometry: VAV3 Antibody [NB300-817]

Flow Cytometry: VAV3 Antibody [NB300-817]

Flow Cytometry: VAV3 Antibody [NB300-817] - Flow cytometric analysis of paraformaldehyde fixed K562 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
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Applications for VAV3 Antibody

Application
Recommended Usage

Flow Cytometry

10 ug/mL

Immunocytochemistry/ Immunofluorescence

10ug/ml

Immunohistochemistry

7.5ug/ml

Immunohistochemistry-Paraffin

7.5 ug/mL

Peptide ELISA

Detection limit 1:32000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: VAV3

VAV3, a third representative of the Vav family, participates in signal transduction processes. It is also involved in the activation of several members of the Rho family. This activity, when deregulated, leads to marked cytoskeletal changes and to alterations in the process of cell division.

Alternate Names

FLJ40431, guanine nucleotide exchange factor VAV3, vav 3 guanine nucleotide exchange factor, vav 3 oncogene, VAV-3

Entrez Gene IDs

10451 (Human)

Gene Symbol

VAV3

UniProt

NP_006104. (Human)

Additional VAV3 Products

Product Documents for VAV3 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for VAV3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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