Key Product Details

Species Reactivity

Human, Mouse, Rat, Canine, Monkey

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI2A3

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all VBP1 Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human VBP1(NP_003363) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

22.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for VBP1 Antibody (OTI2A3) - Azide and BSA Free

Western Blot: VBP1 Antibody (OTI2A3)Azide and BSA Free [NBP2-74833]

Western Blot: VBP1 Antibody (OTI2A3)Azide and BSA Free [NBP2-74833]

Western Blot: VBP1 Antibody (OTI2A3) - Azide and BSA Free [NBP2-74833] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VBP1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VBP1.
Immunocytochemistry/ Immunofluorescence: VBP1 Antibody (OTI2A3) - Azide and BSA Free [NBP2-74833]

Immunocytochemistry/ Immunofluorescence: VBP1 Antibody (OTI2A3) - Azide and BSA Free [NBP2-74833]

Immunocytochemistry/Immunofluorescence: VBP1 Antibody (OTI2A3) - Azide and BSA Free [NBP2-74833] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY VBP1.
Immunohistochemistry: VBP1 Antibody (OTI2A3) - Azide and BSA Free [NBP2-74833]

Immunohistochemistry: VBP1 Antibody (OTI2A3) - Azide and BSA Free [NBP2-74833]

Immunohistochemistry: VBP1 Antibody (OTI2A3) - Azide and BSA Free [NBP2-74833] - Staining of paraffin-embedded Human Kidney tissue using anti-VBP1 mouse monoclonal antibody.
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Applications for VBP1 Antibody (OTI2A3) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Western Blot

1:500-2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: VBP1

VBP1 is encoded by this gene interacts with the Von Hippel-Lindau protein to form an intracellular complex. Because it functions as a chaperone protein, it is suspected that it may play a role in the transport of the Von Hippel-Lindau protein from the perinuclear granules to the nucleus or cytoplasm.

Alternate Names

HIBBJ46, PFD3, PFDN3VHL-binding protein 1, prefoldin 3, prefoldin subunit 3, VBP-1, VHL binding protein-1, von Hippel-Lindau binding protein 1, Von Hippel-Lindau-binding protein 1

Gene Symbol

VBP1

Additional VBP1 Products

Product Documents for VBP1 Antibody (OTI2A3) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for VBP1 Antibody (OTI2A3) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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