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VBP1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-80922

Novus Biologicals, part of Bio-Techne
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NBP1-80922

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: DVDSFMKQPGNETADTVLKKLDEQYQKYKFMELNLAQKKRRLKGQIPEIKQTLEILKYMQKKKESTNSMETRFLLADNLYCKASVPPTDKVCLWLGANVMLEYDIDEAQALLEKNLSTATKNLDSLEEDLDFLRDQFTTTEV

Reactivity Notes

Rat (82%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for VBP1 Antibody

Western Blot: VBP1 Antibody [NBP1-80922]

Western Blot: VBP1 Antibody [NBP1-80922]

Western Blot: VBP1 Antibody [NBP1-80922] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
Immunocytochemistry/ Immunofluorescence: VBP1 Antibody [NBP1-80922]

Immunocytochemistry/ Immunofluorescence: VBP1 Antibody [NBP1-80922]

Immunocytochemistry/Immunofluorescence: VBP1 Antibody [NBP1-80922] - Immunofluorescent staining of human cell line A-431 shows localization to cytosol & vesicles.
Immunohistochemistry-Paraffin: VBP1 Antibody [NBP1-80922]

Immunohistochemistry-Paraffin: VBP1 Antibody [NBP1-80922]

Immunohistochemistry-Paraffin: VBP1 Antibody [NBP1-80922] - Staining of human prostate shows strong cytoplasmic positivity in glandular cells.

Applications for VBP1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: VBP1

VBP1 is encoded by this gene interacts with the Von Hippel-Lindau protein to form an intracellular complex. Because it functions as a chaperone protein, it is suspected that it may play a role in the transport of the Von Hippel-Lindau protein from the perinuclear granules to the nucleus or cytoplasm.

Alternate Names

HIBBJ46, PFD3, PFDN3VHL-binding protein 1, prefoldin 3, prefoldin subunit 3, VBP-1, VHL binding protein-1, von Hippel-Lindau binding protein 1, Von Hippel-Lindau-binding protein 1

Entrez Gene IDs

7411 (Human)

Gene Symbol

VBP1

Additional VBP1 Products

Product Documents for VBP1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for VBP1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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