VCAM-1/CD106 Antibody (1.4C3) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-33182
Clone 1.4C3 was used by HLDA to establish CD designation.
![Immunohistochemistry-Paraffin: VCAM-1/CD106 Antibody (1.4C3) - Azide and BSA Free [NBP2-33182]](https://resources.bio-techne.com/images/products/VCAM-1-CD106-Antibody-1-4C3-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-33182-img0003.jpg "Immunohistochemistry-Paraffin: VCAM-1/CD106 Antibody (1.4C3) - Azide and BSA Free [NBP2-33182]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human
Applications
ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 1.4C3
Format
Azide and BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
Stimulated human umbilical vein endothelial cells (HUVEC)
Localization
Cell surface
Marker
Activated Endothelial Cell Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Theoretical MW
110 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-29413).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for VCAM-1/CD106 Antibody (1.4C3) - Azide and BSA Free
Immunohistochemistry-Paraffin: VCAM-1/CD106 Antibody (1.4C3) - Azide and BSA Free [NBP2-33182]
Immunohistochemistry-Paraffin: VCAM-1/CD106 Antibody (1.4C3) - Azide and BSA Free [NBP2-33182] - Formalin-fixed, paraffin-embedded human tonsil stained with VCAM1 / CD106 antibody at 1:100 using peroxidase-conjugate and DAB chromogen. Note membrane staining.![ELISA: VCAM-1/CD106 Antibody (1.4C3) - Azide and BSA Free [NBP2-33182]](https://resources.bio-techne.com/images/products/VCAM-1-CD106-Antibody-1-4C3-Azide-and-BSA-Free-ELISA-NBP2-33182-img0002.jpg "ELISA: VCAM-1/CD106 Antibody (1.4C3) - Azide and BSA Free [NBP2-33182]")
ELISA: VCAM-1/CD106 Antibody (1.4C3) - Azide and BSA Free [NBP2-33182]
ELISA: VCAM-1/CD106 Antibody (1.4C3) - Azide and BSA Free [NBP2-33182] - Analysis using In-cell ELISA with HDMVEC cells.Applications for VCAM-1/CD106 Antibody (1.4C3) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
1-2 ug/million cells
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Immunohistochemistry-Paraffin
0.5-1ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: VCAM-1/CD106
Long Name
Vascular Cell Adhesion Molecule 1
Alternate Names
CD106, VCAM1
Gene Symbol
VCAM1
Additional VCAM-1/CD106 Products
Product Documents for VCAM-1/CD106 Antibody (1.4C3) - Azide and BSA Free
Product Specific Notices for VCAM-1/CD106 Antibody (1.4C3) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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