VCAM-1/CD106 Antibody (1.4C3) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-33182

Clone 1.4C3 was used by HLDA to establish CD designation.
Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 1.4C3

Format

Azide and BSA Free

Concentration

1.0 mg/ml

View all VCAM-1/CD106 Antibodies »

Product Specifications

Immunogen

Stimulated human umbilical vein endothelial cells (HUVEC)

Localization

Cell surface

Marker

Activated Endothelial Cell Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

110 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-29413).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for VCAM-1/CD106 Antibody (1.4C3) - Azide and BSA Free

Immunohistochemistry-Paraffin: VCAM-1/CD106 Antibody (1.4C3) - Azide and BSA Free [NBP2-33182]

Immunohistochemistry-Paraffin: VCAM-1/CD106 Antibody (1.4C3) - Azide and BSA Free [NBP2-33182]

Immunohistochemistry-Paraffin: VCAM-1/CD106 Antibody (1.4C3) - Azide and BSA Free [NBP2-33182] - Formalin-fixed, paraffin-embedded human tonsil stained with VCAM1 / CD106 antibody at 1:100 using peroxidase-conjugate and DAB chromogen. Note membrane staining.
ELISA: VCAM-1/CD106 Antibody (1.4C3) - Azide and BSA Free [NBP2-33182]

ELISA: VCAM-1/CD106 Antibody (1.4C3) - Azide and BSA Free [NBP2-33182]

ELISA: VCAM-1/CD106 Antibody (1.4C3) - Azide and BSA Free [NBP2-33182] - Analysis using In-cell ELISA with HDMVEC cells.

Applications for VCAM-1/CD106 Antibody (1.4C3) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunohistochemistry-Paraffin

0.5-1ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: VCAM-1/CD106

CD106 is a 110 kD glycosylphosphatidylinositol (GPI)-linked transmembrane protein, also known as VCAM-1 and INCAM-110. It is constitutively expressed on bone marrow stromal cells, myeloid progenitors, splenic dendritic cells, activated endothelial cells, as well as some lymphocytes. CD106 expression can be upregulated on endothelial cells by inflammatory cytokines. CD106 is involved in adhesion and acts as a counter-receptor for VLA-4 ( alpha4/ beta1 integrin) and LPAM-1 ( alpha4/ beta7 integrin). The 429 antibody has been reported to partially block VCAM-1-mediated binding.

Long Name

Vascular Cell Adhesion Molecule 1

Alternate Names

CD106, VCAM1

Gene Symbol

VCAM1

Additional VCAM-1/CD106 Products

Product Documents for VCAM-1/CD106 Antibody (1.4C3) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for VCAM-1/CD106 Antibody (1.4C3) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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