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VCIP135/VCPIP1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-32663

Novus Biologicals, part of Bio-Techne
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NBP1-32663

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse

Predicted:

Rhesus Macaque (99%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockout Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human VCIP135/VCPIP1. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (87%).

Localization

Endoplasmic reticulum, Golgi apparatus, Golgi stack

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

134 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for VCIP135/VCPIP1 Antibody

Western Blot: VCIP135/VCPIP1 Antibody [NBP1-32663]

Western Blot: VCIP135/VCPIP1 Antibody [NBP1-32663]

Western Blot: VCIP135/VCPIP1 Antibody [NBP1-32663] - Wild-type (WT) and VCPIP1 knockout (KO) HeLa cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with VCPIP1 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: VCIP135/VCPIP1 Antibody [NBP1-32663]

Immunocytochemistry/ Immunofluorescence: VCIP135/VCPIP1 Antibody [NBP1-32663]

Immunocytochemistry/Immunofluorescence: VCIP135/VCPIP1 Antibody [NBP1-32663] - HeLa cells were fixed in -20C 100% MeOH for 5 min. Green: VCPIP1 protein stained by VCPIP1 antibody, C-term diluted at 1:500. Blue: Hoechst 33343 staining.
Immunohistochemistry-Paraffin: VCIP135/VCPIP1 Antibody [NBP1-32663]

Immunohistochemistry-Paraffin: VCIP135/VCPIP1 Antibody [NBP1-32663]

Immunohistochemistry-Paraffin: VCIP135/VCPIP1 Antibody [NBP1-32663] - Paraffin-embedded SW480 xenograft, using antibody at 1:100 dilution.

Applications for VCIP135/VCPIP1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: VCIP135/VCPIP1

VCPIP1 - valosin containing protein (p97)/p47 complex interacting protein 1

Long Name

Valosin-Containing Protein P97/P47 Complex-Interacting Protein p135

Alternate Names

DUBA3, VCPIP1

Gene Symbol

VCPIP1

Additional VCIP135/VCPIP1 Products

Product Documents for VCIP135/VCPIP1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for VCIP135/VCPIP1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.