VCIP135/VCPIP1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-32663
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Validated:
Human, Mouse
Predicted:
Rhesus Macaque (99%). Backed by our 100% Guarantee.
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockout Validated, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human VCIP135/VCPIP1. The exact sequence is proprietary.
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Rat (87%).
Localization
Endoplasmic reticulum, Golgi apparatus, Golgi stack
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
134 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for VCIP135/VCPIP1 Antibody
Western Blot: VCIP135/VCPIP1 Antibody [NBP1-32663]
Western Blot: VCIP135/VCPIP1 Antibody [NBP1-32663] - Wild-type (WT) and VCPIP1 knockout (KO) HeLa cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with VCPIP1 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Immunocytochemistry/ Immunofluorescence: VCIP135/VCPIP1 Antibody [NBP1-32663]
Immunocytochemistry/Immunofluorescence: VCIP135/VCPIP1 Antibody [NBP1-32663] - HeLa cells were fixed in -20C 100% MeOH for 5 min. Green: VCPIP1 protein stained by VCPIP1 antibody, C-term diluted at 1:500. Blue: Hoechst 33343 staining.Immunohistochemistry-Paraffin: VCIP135/VCPIP1 Antibody [NBP1-32663]
Immunohistochemistry-Paraffin: VCIP135/VCPIP1 Antibody [NBP1-32663] - Paraffin-embedded SW480 xenograft, using antibody at 1:100 dilution.Applications for VCIP135/VCPIP1 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Immunohistochemistry
1:100-1:1000
Immunohistochemistry-Paraffin
1:100-1:1000
Western Blot
1:500-1:3000
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: VCIP135/VCPIP1
Long Name
Valosin-Containing Protein P97/P47 Complex-Interacting Protein p135
Alternate Names
DUBA3, VCPIP1
Gene Symbol
VCPIP1
Additional VCIP135/VCPIP1 Products
Product Documents for VCIP135/VCPIP1 Antibody
Product Specific Notices for VCIP135/VCPIP1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.