Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # S152B-23

Concentration

1 mg/ml

View all VDAC1 Antibodies »

Product Specifications

Immunogen

Fusion protein amino acids 1-283 (full-length) of human VDAC1. Mouse: 98% identity (279/283 amino acids identical). Rat: 98% identity (279/283 amino acids identical) >60% identity with VDAC2 and VDAC3.

Reactivity Notes

Immunogen displays sequence identity for non-tested species: Mouse and Rat.

Localization

Mitochondrion , Mitochondrion Outer Membrane , Cell Membrane

Specificity

Greater then 60% identity with VDAC2 and VDAC3.Detects approx 30kDa. Does not cross-react with VDAC2 or VDAC3 (based on KO validation results).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for VDAC1 Antibody (S152B-23)

Western Blot: VDAC1 Antibody (S152B-23) [NBP2-42187]

Western Blot: VDAC1 Antibody (S152B-23) [NBP2-42187]

Western Blot: VDAC1 Antibody (S152B-23) [NBP2-42187] - Western Blot analysis of Mouse Brain and Human RT-4 lysates showing detection of ~30 kDa VDAC1 protein using Mouse Anti-VDAC1 Monoclonal Antibody, Clone S152B-23 (NBP2-42187). Lane 1: Molecular Weight Ladder. Lane A: Mouse Brain Lysate. Lane B: Human RT-4 Lysate. Block: 5% Skim milk in TBST . Primary Antibody: Mouse Anti-VDAC1 Monoclonal Antibody (NBP2-42187) at 1:200 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: ~30 kDa.
Immunocytochemistry/ Immunofluorescence: VDAC1 Antibody (S152B-23) [NBP2-42187]

Immunocytochemistry/ Immunofluorescence: VDAC1 Antibody (S152B-23) [NBP2-42187]

Immunocytochemistry/Immunofluorescence: VDAC1 Antibody (S152B-23) [NBP2-42187] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-VDAC1 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Mitochondrion, Mitochondrion Outer Membrane, Nucleus. Magnification: 60X.
Western Blot: VDAC1 Antibody (S152B-23) [NBP2-42187]

Western Blot: VDAC1 Antibody (S152B-23) [NBP2-42187]

Western Blot: VDAC1 Antibody (S152B-23) [NBP2-42187] - Western Blot analysis of Rat Brain Membrane showing detection of ~30 kDa VDAC1 protein using Mouse Anti-VDAC1 Monoclonal Antibody, Clone S152B-23 (NBP2-42187). Lane 1: Molecular Weight Ladder. Lane 2: Rat Brain Membrane. Load: 15 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-VDAC1 Monoclonal Antibody (NBP2-42187) at 1:200 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~30 kDa.

Applications for VDAC1 Antibody (S152B-23)

Application
Recommended Usage

Western Blot

1:1000
Application Notes
1 ug/ml of VDAC1 Antibody was sufficient for detection of VDAC1 in 20 ug of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary Antibody.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 50% Glycerol

Preservative

0.1% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: VDAC1

VDAC1 is an outer membrane mitochondrial protein. The VDAC proteins are thought to form aqueous channels, or pores, through which adenine nucleotides cross the outer mitochondrial membrane. VDACs have been implicated in the formation of the mitochondrial permeability transition pore complex in apoptotic cells. This complex, formed by VDAC, adenine nucleotide translocator (ANT), and cyclophilin D (CypD), is thought to allow the mitochondria to undergo metabolic uncoupling and irreversible morphologic changes that ultimately destroy the mitochondria during apoptosis. VDACs are highly expressed in heart, liver and skeletal muscle, where concentrations of mitochondria are at their highest.

Long Name

Voltage-dependent anion-selective channel protein 1

Alternate Names

hVDAC1, Plasmalemmal porin, Porin 31HL, Porin 31HM, VDAC, VDAC-1

Gene Symbol

VDAC1

UniProt

P21796 (Human)

Additional VDAC1 Products

Product Documents for VDAC1 Antibody (S152B-23)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for VDAC1 Antibody (S152B-23)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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