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VDAC3 Antibody - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-94176

Novus Biologicals, part of Bio-Techne
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NBP2-94176-0.02ml
NBP2-94176-0.1ml

Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-283 of human VDAC3 (NP_005653.3). MCNTPTYCDLGKAAKDVFNKGYGFGMVKIDLKTKSCSGVEFSTSGHAYTDTGKASGNLETKYKVCNYGLTFTQKWNTDNTLGTEISWENKLAEGLKLTLDTIFVPNTGKKSGKLKASYKRDCFSVGSNVDIDFSGPTIYGWAVLAFEGWLAGYQMSFDTAKSKLSQNNFALGYKAADFQLHTHVNDGTEFGGSIYQKVNEKIETSINLAWTAGSNNTRFGIAAKYMLDCRTSLSAKVNNASLIGLGYTQTLRPGVKLTLSALIDGKNFSAGGHKVGLGFELEA

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

30 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for VDAC3 Antibody - Azide and BSA Free

Western Blot: VDAC3 AntibodyAzide and BSA Free [NBP2-94176]

Western Blot: VDAC3 AntibodyAzide and BSA Free [NBP2-94176]

Western Blot: VDAC3 Antibody [NBP2-94176] - Analysis of extracts of Mouse heart, using VDAC3 antibody (NBP2-94176) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.
Immunocytochemistry/ Immunofluorescence: VDAC3 Antibody - Azide and BSA Free [NBP2-94176]

Immunocytochemistry/ Immunofluorescence: VDAC3 Antibody - Azide and BSA Free [NBP2-94176]

Immunocytochemistry/Immunofluorescence: VDAC3 Antibody [NBP2-94176] - Analysis of HeLa cells using VDAC3 . Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: VDAC3 Antibody - Azide and BSA Free [NBP2-94176]

Immunohistochemistry-Paraffin: VDAC3 Antibody - Azide and BSA Free [NBP2-94176]

Immunohistochemistry-Paraffin: VDAC3 Antibody [NBP2-94176] - Mouse lung using VDAC3 Rabbit pAb (NBP2-94176) at dilution of 1:25 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Applications for VDAC3 Antibody - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50 - 1:200

Western Blot

1:100 - 1:500

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

Azide and BSA Free

Preservative

0.05% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: VDAC3

The Voltage-Dependant Anion Channel (VDAC3) of the outer mitochondrial membrane, a small abundant protein found in all eukaryotic kingdoms, forms a voltage-gated pore when incorporated into planar lipid bilayers. VDAC is also the site of binding of the metabolic enzymes hexokinase and glycerol kinase to the mitochondrion in what may be a significant metabolic regulatory interaction. VDAC3 is involved specifically in translocation of adenine nucleotides through the outer membrane.

Alternate Names

HD-VDAC3, hVDAC3, Outer mitochondrial membrane protein porin 3, VDAC-3, voltage-dependent anion channel 3, voltage-dependent anion-selective channel protein 3

Gene Symbol

VDAC3

Additional VDAC3 Products

Product Documents for VDAC3 Antibody - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for VDAC3 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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