Versican Antibody (6D8K9)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15841

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15841-100ul
NBP3-15841-20ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 6D8K9

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Versican Antibodies »

Product Summary for Versican Antibody (6D8K9)

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 3200-3396 of human Versican (P13611). QGAHLTSILSHEEQMFVNRVGHDYQWIGLNDKMFEHDFRWTDGSTLQYENWRPNQPDSFFSAGEDCVVIIWHENGQWNDVPCNYHLTYTCKKGTVACGQPPVVENAKTFGKMKPRYEINSLIRYHCKDGFIQRHLPTIRCLGNGRWAIPKITCMNPSAYQRTYSMKYFKNSSSAKDNSINTSKHDHRWSRRWQESRR

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

373 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Versican Antibody (6D8K9)

Western Blot: Versican Antibody (6D8K9) [NBP3-15841]

Western Blot: Versican Antibody (6D8K9) [NBP3-15841]

Western Blot: Versican Antibody (6D8K9) [NBP3-15841] - Analysis of extracts of various cell lines, using Versican antibody (NBP3-15841) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.
Immunohistochemistry-Paraffin: Versican Antibody (6D8K9) [NBP3-15841]

Immunohistochemistry-Paraffin: Versican Antibody (6D8K9) [NBP3-15841]

Immunohistochemistry-Paraffin: Versican Antibody (6D8K9) [NBP3-15841] - Mouse brain using Versican Rabbit mAb (NBP3-15841) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence: Versican Antibody (6D8K9) [NBP3-15841]

Immunocytochemistry/ Immunofluorescence: Versican Antibody (6D8K9) [NBP3-15841]

Immunocytochemistry/Immunofluorescence: Versican Antibody (6D8K9) [NBP3-15841] - Immunofluorescence analysis of C6 cells using Versican Rabbit mAb (NBP3-15841) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Applications for Versican Antibody (6D8K9)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 -1:200

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Versican

Versican is a member of the aggrecan/versican proteoglycan family. The protein encoded is a large chondroitin sulfate proteoglycan and is a major component of the extracellular matrix. This protein is involved in cell adhesion, proliferation, proliferation, migration and angiogenesis and plays a central role in tissue morphogenesis and maintenance. Mutations in this gene are the cause of Wagner syndrome type 1. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Alternate Names

CSPG2, GHAP, PG-M, VCAN

Gene Symbol

VCAN

Additional Versican Products

Product Documents for Versican Antibody (6D8K9)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Versican Antibody (6D8K9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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