Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # S351-23

Concentration

1 mg/ml

View all Versican Antibodies »

Product Specifications

Immunogen

Fusion protein amino acids 362-585 (glycosaminoglycan alpha domain) of mouse Versican core protein

Localization

Extracellular Space , Extracellular Matrix

Specificity

Detects >350kDa.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Versican Antibody (S351-23)

Western Blot: Versican Antibody (S351-23) [NBP2-22408]

Western Blot: Versican Antibody (S351-23) [NBP2-22408]

Western Blot: Versican Antibody (S351-23) [NBP2-22408] - Western Blot analysis of Rat Brain Membrane and brain showing detection of 350kDa Versicanprotein using Mouse Anti-Versican Monoclonal Antibody, Clone S351-23 (NBP2-22408). Lane 1: Molecular Weight Ladder. Lane 2: Rat Brain Membrane and brain. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-Versican Monoclonal Antibody (NBP2-22408) at 1:200 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: KPL TMB. Predicted/Observed Size: 350kDa. Other Band(s): Multiple other bands.
Immunocytochemistry/ Immunofluorescence: Versican Antibody (S351-23) [NBP2-22408]

Immunocytochemistry/ Immunofluorescence: Versican Antibody (S351-23) [NBP2-22408]

Immunocytochemistry/Immunofluorescence: Versican Antibody (S351-23) [NBP2-22408] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-VersicanMonoclonal Antibody, Clone S351-23 (NBP2-22408). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-VersicanMonoclonal Antibody (NBP2-22408) at 1:200 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) VersicanAntibody (D) Composite.
Immunocytochemistry/ Immunofluorescence: Versican Antibody (S351-23) [NBP2-22408]

Immunocytochemistry/ Immunofluorescence: Versican Antibody (S351-23) [NBP2-22408]

Immunocytochemistry/Immunofluorescence: Versican Antibody (S351-23) [NBP2-22408] - Analysis using Mouse Anti-Versican Monoclonal Antibody, Clone S351-23. Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Versican Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm, Membrane, Extracellular Space, Extracellular Matrix. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) Versican Antibody (D) Composite.
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Applications for Versican Antibody (S351-23)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:1000
Application Notes
1 ug/ml of Versican Antibody was sufficient for detection of Versican in 20 ug of mouse brain membrane lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary Antibody.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 50% Glycerol

Preservative

0.1% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Versican

Versican is a member of the aggrecan/versican proteoglycan family. The protein encoded is a large chondroitin sulfate proteoglycan and is a major component of the extracellular matrix. This protein is involved in cell adhesion, proliferation, proliferation, migration and angiogenesis and plays a central role in tissue morphogenesis and maintenance. Mutations in this gene are the cause of Wagner syndrome type 1. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Alternate Names

CSPG2, GHAP, PG-M, VCAN

Gene Symbol

VCAN

UniProt

Q62059 (Mouse)

Additional Versican Products

Product Documents for Versican Antibody (S351-23)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Versican Antibody (S351-23)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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