VGluT3 Antibody (S34-34)

Immunocytochemistry/ Immunofluorescence: VGluT3 Antibody (S34-34) [NBP2-59331]

Immunocytochemistry/Immunofluorescence: VGluT3 Antibody (S34-34) [NBP2-59331] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-VGluT3 Monoclonal Antibody, Clone S34-34 (NBP2-59331). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-VGluT3 Monoclonal Antibody (NBP2-59331) at 1:50 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) VGluT3 Antibody (D) Composite.

Immunocytochemistry/ Immunofluorescence: VGluT3 Antibody (S34-34) [NBP2-59331]

Immunocytochemistry/Immunofluorescence: VGluT3 Antibody (S34-34) [NBP2-59331] - VGlut3 Antibody (S34-34) [NBP2-59331] - Analysis using Mouse Anti-VGlut3 Monoclonal Antibody, Clone S34-34. Tissue: SK-N-BE Cells (Human Neuroblastoma cells). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-VGlut3 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasmic Vesicle, Secretory Vesicle, Synaptic Vesicle Membrane, Membrane, Cell Junction, Synapse. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) VGlut3 Antibody (D) Composite.