VHR/DUSP3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-98873

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

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Product Summary for VHR/DUSP3 Antibody

Immunogen

Produced in rabbits immunized with purified, recombinant Human VHR/DUSP3 (Accession#: P51452; Met1-Pro185)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for VHR/DUSP3 Antibody

Western Blot: VHR/DUSP3 Antibody [NBP2-98873]

Western Blot: VHR/DUSP3 Antibody [NBP2-98873]

Western Blot: VHR/DUSP3 Antibody [NBP2-98873] - Anti-VHR/DUSP3 rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 20 kDa. Observed band size: 22 kDa
Immunoprecipitation: VHR/DUSP3 Antibody [NBP2-98873]

Immunoprecipitation: VHR/DUSP3 Antibody [NBP2-98873]

Immunoprecipitation: VHR/DUSP3 Antibody [NBP2-98873] - VHR/DUSP3 was immunoprecipitated using: Lane A: 0.5 mg HepG2 Whole Cell Lysate. Lane B: 0.5 mg Hela Whole Cell Lysate. Lane C: 0.5 mg 293T Whole Cell Lysate. 1 ul anti-VHR/DUSP3 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-VHR/DUSP3 rabbit polyclonal antibody, at 1:500 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 20 kDa. Observed band size: 20 kDa

Applications for VHR/DUSP3 Antibody

Application
Recommended Usage

ELISA

1:25000-1:50000

Immunoprecipitation

0.2-1 uL/mg of lysate

Western Blot

1:500-1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: VHR

DUSP3 is encoded by this gene is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which are associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. This gene maps in a region that contains the BRCA1 locus which confers susceptibility to breast and ovarian cancer. Although DUSP3 is expressed in both breast and ovarian tissues, mutation screening in breast cancer pedigrees and in sporadic tumors was negative, leading to the conclusion that this gene is not BRCA1.

Long Name

Vaccinia Virus VH1-related Phosphatase

Alternate Names

DUSP3

Gene Symbol

DUSP3

Additional VHR Products

Product Documents for VHR/DUSP3 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for VHR/DUSP3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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