Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Protein Array

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # rVIL1/1325

Format

Azide and BSA Free

Concentration

1 mg/ml

View all Villin 1 Antibodies »

Product Specifications

Immunogen

A recombinant fragment (around aa179-311) of human Villin 1 protein (exact sequence is proprietary) (Uniprot: P09327)

Localization

Cytoplasmic & Cell Surface

Specificity

Recognizes a protein of 95kDa, which is identified as villin. It is a major constituent in the microvilli, which compose the brush border of epithelial cells forming absorptive surfaces of the intestinal and renal proximal tubular epithelia. Anti-Villin labels the brush border area in the gastrointestinal mucosal epithelium and urogenital tract. Among neoplasms, villin is predominantly expressed in tumors of colorectal origin. Antibody to villin is useful in identifying malignant cells from primary and metastatic colorectal carcinomas. This antibody also labels Merkel cells of the skin.

Marker

GI-Mucosal & Urogenital Brush Border Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

93 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-79809).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Villin 1 Antibody (rVIL1/1325) - Azide and BSA Free

Immunohistochemistry-Paraffin: Villin 1 Antibody (rVIL1/1325) - Azide and BSA Free [NBP2-79943]

Immunohistochemistry-Paraffin: Villin 1 Antibody (rVIL1/1325) - Azide and BSA Free [NBP2-79943]

Immunohistochemistry-Paraffin: Villin 1 Antibody (rVIL1/1325) - Azide and BSA Free [NBP2-79943] - Formalin-fixed, paraffin-embedded human Small Intestinal Carcinoma stained with Villin-Monospecific Recombinant Mouse Monoclonal Antibody (rVIL1/1325).
Immunohistochemistry-Paraffin: Villin 1 Antibody (rVIL1/1325) - Azide and BSA Free [NBP2-79943]

Immunohistochemistry-Paraffin: Villin 1 Antibody (rVIL1/1325) - Azide and BSA Free [NBP2-79943]

Immunohistochemistry-Paraffin: Villin 1 Antibody (rVIL1/1325) - Azide and BSA Free [NBP2-79943] - Formalin-fixed, paraffin-embedded human Small Intestinal Carcinoma stained with Villin-Monospecific Recombinant Mouse Monoclonal Antibody (rVIL1/1325).
Protein Array: Villin 1 Antibody (rVIL1/1325)Azide and BSA Free [NBP2-79943]

Protein Array: Villin 1 Antibody (rVIL1/1325)Azide and BSA Free [NBP2-79943]

Protein Array: Villin 1 Antibody (rVIL1/1325) - Azide and BSA Free [NBP2-79943] - Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

Applications for Villin 1 Antibody (rVIL1/1325) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1 - 2 ug/mL

Immunohistochemistry-Paraffin

1 - 2 ug/mL
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: Villin 1

Villin 1 encodes a member of a family of calcium-regulated actin-binding proteins. This protein represents a dominant part of the brush border cytoskeleton which functions in the capping, severing, and bundling of actin filaments. Two mRNAs of 2.7 kb and 3.5 kb have been observed; they result from utilization of alternate poly-adenylation signals present in the terminal exon. [provided by RefSeq]

Alternate Names

D2S1471, VIL, villin 1, villin-1

Gene Symbol

VIL1

Additional Villin 1 Products

Product Documents for Villin 1 Antibody (rVIL1/1325) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Villin 1 Antibody (rVIL1/1325) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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