Villin 1 Antibody (rVIL1/8336) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-24212
Recombinant Monoclonal Antibody
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # rVIL1/8336
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Recombinant fragment (around aa600-700) of human Villin 1 protein (exact sequence is proprietary)
Localization
Cytoplasm. Cell Surface.
Specificity
Anti-Villin labels the brush border area in the gastrointestinal mucosal epithelium and urogenital tract. Among neoplasms, villin is predominantly expressed in tumors of colorectal origin.Antibody to villin is useful in identifying malignant cells from primary and metastatic colorectal carcinomas. This antibody also labels Merkel cells of the skin.
Marker
GI-Mucosal & Urogenital Brush Border Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
Antibody without azide - store at -20 to -80C.
Scientific Data Images for Villin 1 Antibody (rVIL1/8336) - Azide and BSA Free
Immunohistochemistry-Paraffin: Villin 1 Antibody (rVIL1/8336) - Azide and BSA Free [NBP3-24212] -
Formalin-fixed, paraffin-embedded human colon adenocarcinoma stained with Villin 1 antibody (rVIL1/8336) - Azide and BSA Free. HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.Applications for Villin 1 Antibody (rVIL1/8336) - Azide and BSA Free
Application
Recommended Usage
Immunohistochemistry-Paraffin
1-2ug/ml
Application Notes
Immunohistochemistry-Paraffin: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: Villin 1
Alternate Names
D2S1471, VIL, villin 1, villin-1
Gene Symbol
VIL1
Additional Villin 1 Products
Product Documents for Villin 1 Antibody (rVIL1/8336) - Azide and BSA Free
Product Specific Notices for Villin 1 Antibody (rVIL1/8336) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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