Villin 1 Antibody (VIL1/1314 + VIL1/2376)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-07176
![Immunohistochemistry-Paraffin: Villin 1 Antibody (VIL1/1314 + VIL1/2376) [NBP3-07176]](https://resources.bio-techne.com/images/products/Villin-1-Antibody-VIL1-1314-+-VIL1-2376-Immunohistochemistry-Paraffin-NBP3-07176-img0002.jpg "Immunohistochemistry-Paraffin: Villin 1 Antibody (VIL1/1314 + VIL1/2376) [NBP3-07176]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Kappa/IgG1 Kappa Clone # VIL1/1314 + VIL1/2376
Concentration
0.2 mg/ml
Product Specifications
Immunogen
A recombinant fragment (around aa179-311) of human Villin 1 protein (exact sequence is proprietary) (Uniprot: P09327)
Localization
Cytoplasmic & Cell Surface
Specificity
Recognizes a protein of 95kDa, which is identified as villin. It is a major constituent in the microvilli, which compose the brush border of epithelial cells forming absorptive surfaces of the intestinal and renal proximal tubular epithelia. Anti-Villin labels the brush border area in the gastrointestinal mucosal epithelium and urogenital tract. Among neoplasms, villin is predominantly expressed in tumors of colorectal origin. Antibody to villin is useful in identifying malignant cells from primary and metastatic colorectal carcinomas. This antibody also labels Merkel cells of the skin.
Marker
GI-Mucosal & Urogenital Brush Border Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 Kappa/IgG1 Kappa
Theoretical MW
93 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08919)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for Villin 1 Antibody (VIL1/1314 + VIL1/2376)
Immunohistochemistry-Paraffin: Villin 1 Antibody (VIL1/1314 + VIL1/2376) [NBP3-07176]
Immunohistochemistry-Paraffin: Villin 1 Antibody (VIL1/1314 + VIL1/2376) [NBP3-07176] - Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Villin 1 Mouse Monoclonal Antibody (VIL1/1314 + VIL1/2376).![Immunohistochemistry-Paraffin: Villin 1 Antibody (VIL1/1314 + VIL1/2376) [NBP3-07176]](https://resources.bio-techne.com/images/products/Villin-1-Antibody-VIL1-1314-+-VIL1-2376-Immunohistochemistry-Paraffin-NBP3-07176-img0001.jpg "Immunohistochemistry-Paraffin: Villin 1 Antibody (VIL1/1314 + VIL1/2376) [NBP3-07176]")
Immunohistochemistry-Paraffin: Villin 1 Antibody (VIL1/1314 + VIL1/2376) [NBP3-07176]
Immunohistochemistry-Paraffin: Villin 1 Antibody (VIL1/1314 + VIL1/2376) [NBP3-07176] - Formalin-fixed, paraffin-embedded human Small Intestine stained with Villin 1 Mouse Monoclonal Antibody (VIL1/1314 + VIL1/2376).Applications for Villin 1 Antibody (VIL1/1314 + VIL1/2376)
Application
Recommended Usage
Immunohistochemistry-Paraffin
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: Villin 1
Additional Villin 1 Products
Product Documents for Villin 1 Antibody (VIL1/1314 + VIL1/2376)
Product Specific Notices for Villin 1 Antibody (VIL1/1314 + VIL1/2376)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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