Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # VIL1/1325

Concentration

0.2 mg/ml

View all Villin 1 Antibodies »

Product Specifications

Immunogen

Recombinant human Villin 1 fragment (around aa179-311) (exact sequence is proprietary)) (Uniprot: P09327)

Localization

Cytoplasmic & Cell Surface

Specificity

Recognizes a protein of 95kDa, which is identified as villin. It is a major constituent in the microvilli, which compose the brush border of epithelial cells forming absorptive surfaces of the intestinal and renal proximal tubular epithelia. Anti-Villin labels the brush border area in the gastrointestinal mucosal epithelium and urogenital tract. Among neoplasms, villin is predominantly expressed in tumors of colorectal origin. Antibody to villin is useful in identifying malignant cells from primary and metastatic colorectal carcinomas. This antibody also labels Merkel cells of the skin.

Marker

GI-Mucosal & Urogenital Brush Border Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

93 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-54515)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Villin 1 Antibody (VIL1/1325)

Western Blot: Villin 1 Antibody (VIL1/1325) [NBP2-53201]

Western Blot: Villin 1 Antibody (VIL1/1325) [NBP2-53201]

Western Blot: Villin 1 Antibody (VIL1/1325) [NBP2-53201] - Western Blot of A549, HepG2 & HCT116 Cell Lysates with Villin Monoclonal Antibody (VIL1/1325).
Immunohistochemistry-Paraffin: Villin 1 Antibody (VIL1/1325) [NBP2-53201]

Immunohistochemistry-Paraffin: Villin 1 Antibody (VIL1/1325) [NBP2-53201]

Immunohistochemistry-Paraffin: Villin 1 Antibody (VIL1/1325) [NBP2-53201] - Formalin-fixed, paraffin-embedded human Rectum stained with Villin Monoclonal Antibody (VIL1/1325).
Immunohistochemistry-Paraffin: Villin 1 Antibody (VIL1/1325) [NBP2-53201]

Immunohistochemistry-Paraffin: Villin 1 Antibody (VIL1/1325) [NBP2-53201]

Immunohistochemistry-Paraffin: Villin 1 Antibody (VIL1/1325) [NBP2-53201] - Formalin-fixed, paraffin-embedded human Colon stained with Villin Monoclonal Antibody (VIL1/1325).

Applications for Villin 1 Antibody (VIL1/1325)

Application
Recommended Usage

Immunohistochemistry-Paraffin

0.25-0.5 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 0.25-0.5ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Western Blot: 1-2ug/ml for 60 minutes at RT.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Villin 1

Villin 1 encodes a member of a family of calcium-regulated actin-binding proteins. This protein represents a dominant part of the brush border cytoskeleton which functions in the capping, severing, and bundling of actin filaments. Two mRNAs of 2.7 kb and 3.5 kb have been observed; they result from utilization of alternate poly-adenylation signals present in the terminal exon. [provided by RefSeq]

Alternate Names

D2S1471, VIL, villin 1, villin-1

Gene Symbol

VIL1

UniProt

P09327 (Human)

Additional Villin 1 Products

Product Documents for Villin 1 Antibody (VIL1/1325)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Villin 1 Antibody (VIL1/1325)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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