Villin 1 Antibody (VIL1/7378R)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-20321
Recombinant Monoclonal Antibody
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Kappa Clone # VIL1/7378R
Concentration
0.2 mg/ml
Product Specifications
Immunogen
Recombinant fragment (around aa600-700) of human Villin 1 protein (exact sequence is proprietary)
Localization
Cytoplasm. Cell Surface.
Specificity
Anti-Villin 1 labels the brush border area in the gastrointestinal mucosal epithelium and urogenital tract This antibody also labels Merkel cells of the skin
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG Kappa
Description
Positive Controls: A549, HepG2 or HCT116 cells. Human colon or rectum.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Non-hazardous. No MSDS required.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Non-hazardous. No MSDS required.
Scientific Data Images for Villin 1 Antibody (VIL1/7378R)
Villin 1 Antibody (VIL1/7378R)
Formalin-fixed, paraffin-embedded human colon adenocarcinoma stained with Villin 1 antibody (VIL1/7378R). HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.
Villin 1 Antibody (VIL1/7378R)
Formalin-fixed, paraffin-embedded human colon adenocarcinoma stained with Villin 1 antibody (VIL1/7378R). Inset: PBS instead of primary antibody; secondary only negative control.Applications for Villin 1 Antibody (VIL1/7378R)
Application
Recommended Usage
Immunohistochemistry-Paraffin
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes. at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: Villin 1
Alternate Names
D2S1471, VIL, villin 1, villin-1
Gene Symbol
VIL1
Additional Villin 1 Products
Product Documents for Villin 1 Antibody (VIL1/7378R)
Product Specific Notices for Villin 1 Antibody (VIL1/7378R)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...