Vinculin Antibody (3F8-1D4)

Novus Biologicals, part of Bio-Techne | Catalog # H00007414-M02

Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
H00007414-M02
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 3F8-1D4

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Vinculin Antibodies »

Product Specifications

Immunogen

VCL (AAH39174.1, 1 a.a. ~ 1066 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MPVFHTRTIESILEPVAQQISHLVIMHEEGEVDGKAIPDLTAPVAAVQAAVSNLVRVGKETVQTTEDQILKRDMPPAFIKVENACTKLVQAAQMLQSDPYSVPARDYLIDGSRGILSGTSDLLLTFDEAEVRKIIRVCKGILEYLTVAEVVETMEDLVTYTKNLGPGMTKMAKMIDERQQELTHQEHRVMLVNSMNTVKELLPVLISAMKIFVTTKNSKNQGIEEALKNRNFTLEKMSAEINEIIRVLQLTSWDEDAWASKDTEAMKRALASIDSKLNQAKGWLRDPSASPGDAGEQAIRQILDEAGKVGELCAGKERREILGTCKMLGQMTDQVADLRARGQGSSPVAMQKAQQVSQGLDVLTAKVENAARKLEAMTNSKQSIAKKIDAAQNWLADPNGGPEGEEQIRGALAEARKIAELCDDPKERDDILRSLGEISALTSKLADLRRQGKGDSPEARALAKQVATALQNLQTKTNRAVANSRPAKAAVHLEGKIEQAQRWIDNPTVDDRGVGQAAIRGLVAEGHRLANVMMGPYRQDLLAKCDRVDQLTAQLADLAARGEGESPQARALASQLQDSLKDLKARMQEAMTQEVSDVFSDTTTPIKLLAVAATAPPDAPNREEVFDERAANFENHSGKLGATAEKAAAVGTANKSTVEGIQASVKTARELTPQVVSAARILLRNPGNQAAYEHFETMKNQWIDNVEKMTGLVDEAIDTKSLLDASEEAIKKDLDKCKVAMANIQPQMLVAGATSIARRANRILLVAKREVENSEDPKFREAVKAASDELSKTISPMVMDAKAVAGNISDPGLQKSFLDSGYRILGAVAKVREAFQPQEPDFPPPPPDLEQLRLTDELAPPKPPLPEGEVPPPRPPPPEEKDEEFPEQKAGEVINQPMMMAARQLHDEARKWSSKGNDIIAAAKRMALLMAEMSRLVRGGSGTKRALIQCAKDIAKASDEVTRLAKEVAKQCTDKRIRTNLLQVCERIPTISTQLKILSTVKATMLGRTNISDEESEQATEMLVHNAQNLMQSVKETVREAEAASIKIRTDAGFTLRWVRKTPWYQ

Specificity

VCL - vinculin (3F8-1D4)

Marker

Focal Adhesion Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Vinculin Antibody (3F8-1D4)

Western Blot: Vinculin Antibody (3F8-1D4) [H00007414-M02]

Western Blot: Vinculin Antibody (3F8-1D4) [H00007414-M02]

Western Blot: Vinculin Antibody (3F8-1D4) [H00007414-M02] - Vinculin monoclonal antibody, clone 3F8-1D4. Western Blot analysis of VCL expression in HeLa.
Immunocytochemistry/ Immunofluorescence: Vinculin Antibody (3F8-1D4) [H00007414-M02]

Immunocytochemistry/ Immunofluorescence: Vinculin Antibody (3F8-1D4) [H00007414-M02]

Immunocytochemistry/Immunofluorescence: Vinculin Antibody (3F8-1D4) [H00007414-M02] - Analysis of monoclonal antibody to VCL on HeLa cell . Antibody concentration 10 ug/ml.
ELISA: Vinculin Antibody (3F8-1D4) [H00007414-M02]

ELISA: Vinculin Antibody (3F8-1D4) [H00007414-M02]

ELISA: Vinculin Antibody (3F8-1D4) [H00007414-M02] - Detection limit for recombinant GST tagged VCL is approximately 0.1ng/ml as a capture antibody.

Applications for Vinculin Antibody (3F8-1D4)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Vinculin

Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Defects in VCL are the cause of cardiomyopathy dilated type 1W. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined. [provided by RefSeq]

Long Name

Metavinculin

Alternate Names

MVCL, VCL

Entrez Gene IDs

7414 (Human)

Gene Symbol

VCL

UniProt

AAH39174 (Human)

Additional Vinculin Products

Product Documents for Vinculin Antibody (3F8-1D4)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Vinculin Antibody (3F8-1D4)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.