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Vinculin Antibody (hVIN-1)

Novus Biologicals, part of Bio-Techne | Catalog # NB600-1293

Novus Biologicals, part of Bio-Techne
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NB600-1293
NB600-1293-0.025ml

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat, Amphibian, Bovine, Canine, Chicken, Turkey

Cited:

Human, Mouse, Rat

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Simple Western, Single Cell Western, Western Blot

Cited:

IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Frozen, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # hVIN-1

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Product Specifications

Immunogen

Purified human vinculin from uterus.

Reactivity Notes

Frog (100%). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Localization

Cytoplasm; cytoskeleton. Cell junction; adherens junction; cytoplasmic side. Cytoplasmic face of adhesion plaques.

Specificity

Specifically labels vinculin at cell-cell and cell-substrate contacts. Shows cross-reactivity with smooth muscle metavinculin.

Marker

Focal Adhesion Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

116 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Vinculin Antibody (hVIN-1)

Western Blot: Vinculin Antibody (hVIN-1) [NB600-1293]

Western Blot: Vinculin Antibody (hVIN-1) [NB600-1293]

Western Blot: Vinculin Antibody (hVIN-1) [NB600-1293] - Cell line lysates were separated on SDS-PAGE and probed with 1:200 Monoclonal Anti-Vinculin Clone: hVIN-1. The antibody was developed using Goat Anti-Mouse IgG-Peroxidase and a chemiluminescent substrate.Lanes:1.HeLa2.COS73.NIH-3T34.RAT25.CHO6.MDBK7.MDCK
Immunocytochemistry/ Immunofluorescence: Vinculin Antibody (hVIN-1) [NB600-1293]

Immunocytochemistry/ Immunofluorescence: Vinculin Antibody (hVIN-1) [NB600-1293]

Immunocytochemistry/Immunofluorescence: Vinculin Antibody (hVIN-1) [NB600-1293] - Immunofluorescence images of LM8 cells treated with 0 nM, 1 nM, or 10 nM eribulin and stained for vinculin (red) and nucleus (blue) (left). Dotted line shows the cell shape. Scale bar: 10 um. Quantitative analysis of the area of vinculin staining (right). Values are mean +/- SEM (less than or equal to 30 cells per group). **P < 0.01. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30719211/) licensed under a CC-BY license.
Immunohistochemistry: Vinculin Antibody (hVIN-1) [NB600-1293]

Immunohistochemistry: Vinculin Antibody (hVIN-1) [NB600-1293]

Immunohistochemistry: Vinculin Antibody (hVIN-1) [NB600-1293] - Enhanced Validation-By Independent Antibodies:Immunohistochemistry. Formalin-fixed, paraffin-embedded Rat Heart sections stained with 15 ug/mL Anti-Vinculin antibody produced in Rabbit (Cat. No. V4139) (A). The antibody was developed using Anti-Rabbit IgG (whole molecule)-FITC antibody produced in Goat (Cat. No. F9887), and 15 ug/mL Monoclonal Anti-Vinculin antibody produced in Mouse, Clone: hVIN1 (Cat. No. V9131) (B). The antibody was developed using Rabbit Anti-Mouse IgG-Cy3 conjugate antibody.Results:Two Anti-Vinculin antibodies, V4139 (A) and V9131 (B), target different regions of Vinculin show similar staining profiles between the two antibodies, demonstrating Independent Antibody Verification.

Applications for Vinculin Antibody (hVIN-1)

Application
Recommended Usage

Immunohistochemistry

1:10 - 1:500

Immunohistochemistry-Frozen

1:10 - 1:500

Single Cell Western

1:30

Western Blot

1:200 - 1:400
Application Notes
See Simple Western Antibody Database for Simple Western validation: tested in HeLa lysate; separated by size; antibody dilution of 1:5; matrix was 12-230 kDa. Single Cell Western reported by an internal validation on treated LNCap cells at a 1:30 dilution

Reviewed Applications

Read 1 review rated 5 using NB600-1293 in the following applications:

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Ascites

Preservative

0.9% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Vinculin

Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Defects in VCL are the cause of cardiomyopathy dilated type 1W. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined. (provided by RefSeq)

Long Name

Metavinculin

Alternate Names

MVCL, VCL

Entrez Gene IDs

7414 (Human); 22330 (Mouse); 305679 (Rat)

Gene Symbol

VCL

UniProt

Additional Vinculin Products

Product Documents for Vinculin Antibody (hVIN-1)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Vinculin Antibody (hVIN-1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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