Vinculin Antibody (hVIN-1)
Novus Biologicals, part of Bio-Techne | Catalog # NB600-1293
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human, Mouse, Rat, Amphibian, Bovine, Canine, Chicken, Turkey
Cited:
Human, Mouse, Rat
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Simple Western, Single Cell Western, Western Blot
Cited:
IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Frozen, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # hVIN-1
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Product Specifications
Immunogen
Purified human vinculin from uterus.
Reactivity Notes
Frog (100%). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Localization
Cytoplasm; cytoskeleton. Cell junction; adherens junction; cytoplasmic side. Cytoplasmic face of adhesion plaques.
Specificity
Specifically labels vinculin at cell-cell and cell-substrate contacts. Shows cross-reactivity with smooth muscle metavinculin.
Marker
Focal Adhesion Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
116 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Vinculin Antibody (hVIN-1)
Western Blot: Vinculin Antibody (hVIN-1) [NB600-1293]
Western Blot: Vinculin Antibody (hVIN-1) [NB600-1293] - Cell line lysates were separated on SDS-PAGE and probed with 1:200 Monoclonal Anti-Vinculin Clone: hVIN-1. The antibody was developed using Goat Anti-Mouse IgG-Peroxidase and a chemiluminescent substrate.Lanes:1.HeLa2.COS73.NIH-3T34.RAT25.CHO6.MDBK7.MDCKImmunocytochemistry/ Immunofluorescence: Vinculin Antibody (hVIN-1) [NB600-1293]
Immunocytochemistry/Immunofluorescence: Vinculin Antibody (hVIN-1) [NB600-1293] - Immunofluorescence images of LM8 cells treated with 0 nM, 1 nM, or 10 nM eribulin and stained for vinculin (red) and nucleus (blue) (left). Dotted line shows the cell shape. Scale bar: 10 um. Quantitative analysis of the area of vinculin staining (right). Values are mean +/- SEM (less than or equal to 30 cells per group). **P < 0.01. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30719211/) licensed under a CC-BY license.Immunohistochemistry: Vinculin Antibody (hVIN-1) [NB600-1293]
Immunohistochemistry: Vinculin Antibody (hVIN-1) [NB600-1293] - Enhanced Validation-By Independent Antibodies:Immunohistochemistry. Formalin-fixed, paraffin-embedded Rat Heart sections stained with 15 ug/mL Anti-Vinculin antibody produced in Rabbit (Cat. No. V4139) (A). The antibody was developed using Anti-Rabbit IgG (whole molecule)-FITC antibody produced in Goat (Cat. No. F9887), and 15 ug/mL Monoclonal Anti-Vinculin antibody produced in Mouse, Clone: hVIN1 (Cat. No. V9131) (B). The antibody was developed using Rabbit Anti-Mouse IgG-Cy3 conjugate antibody.Results:Two Anti-Vinculin antibodies, V4139 (A) and V9131 (B), target different regions of Vinculin show similar staining profiles between the two antibodies, demonstrating Independent Antibody Verification.Applications for Vinculin Antibody (hVIN-1)
Application
Recommended Usage
Immunohistochemistry
1:10 - 1:500
Immunohistochemistry-Frozen
1:10 - 1:500
Single Cell Western
1:30
Western Blot
1:200 - 1:400
Application Notes
See Simple Western Antibody Database for Simple Western validation: tested in HeLa lysate; separated by size; antibody dilution of 1:5; matrix was 12-230 kDa. Single Cell Western reported by an internal validation on treated LNCap cells at a 1:30 dilution
Reviewed Applications
Read 1 review rated 5 using NB600-1293 in the following applications:
Formulation, Preparation, and Storage
Purification
Unpurified
Formulation
Ascites
Preservative
0.9% Sodium Azide
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Vinculin
Long Name
Metavinculin
Alternate Names
MVCL, VCL
Gene Symbol
VCL
UniProt
Additional Vinculin Products
Product Documents for Vinculin Antibody (hVIN-1)
Product Specific Notices for Vinculin Antibody (hVIN-1)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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