Vinculin Antibody (VCL/8533R) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-21067
Recombinant Monoclonal Antibody
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Mouse
Applications
Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Kappa Clone # VCL/8533R
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Recombinant fragment (around aa 174-322) of human Vinculin protein (exact sequence is proprietary)
Localization
Cytoplasm
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG Kappa
Description
Positive Controls: A431, K562, NIH3T3, U-87 or THP-1 cells. Human bladder or testis.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Non-hazardous. No MSDS required.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Non-hazardous. No MSDS required.
Scientific Data Images for Vinculin Antibody (VCL/8533R) - Azide and BSA Free
Vinculin Antibody (VCL/8533R)
Formalin-fixed, paraffin-embedded human kidney stained with Vinculin antibody (VCL/8533R). Inset: PBS instead of primary antibody; secondary only negative control.Applications for Vinculin Antibody (VCL/8533R) - Azide and BSA Free
Application
Recommended Usage
Immunohistochemistry-Paraffin
1-2 ug/ml
Western Blot
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes. at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -70C. Avoid freeze-thaw cycles.
Background: Vinculin
Long Name
Metavinculin
Alternate Names
MVCL, VCL
Gene Symbol
VCL
Additional Vinculin Products
Product Documents for Vinculin Antibody (VCL/8533R) - Azide and BSA Free
Product Specific Notices for Vinculin Antibody (VCL/8533R) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...