Viral Chikungunya Virus E1 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB9779

R&D Systems, part of Bio-Techne
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MAB9779-100
MAB9779-SP
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Key Product Details

Species Reactivity

Viral

Applications

ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 988110

View all Chikungunya Virus E1 Antibodies »

Product Specifications

Immunogen

Human embryonic kidney cell line HEK293-derived transfected with viral Chikungunya Virus E1
Tyr810-Gly1224
Accession # Q8JUX5

Specificity

Detects viral Chikungunya Virus E1 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Viral Chikungunya Virus E1 Antibody

Viral Chikungunya Virus E1 Antibody in ELISA Standard Curve.

Viral Chikungunya Virus E1 ELISA Standard Curve.

Recombinant Viral Chikungunya Virus E1 protein was serially diluted 2-fold and captured by Mouse Anti-Viral Chikungunya Virus E1 Monoclonal Antibody (Catalog # MAB97791) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Viral Chikungunya Virus E1 Monoclonal Antibody (Catalog # MAB9779) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Applications for Viral Chikungunya Virus E1 Antibody

Application
Recommended Usage

ELISA

This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Viral Chikungunya Virus E1 Monoclonal Antibody (Catalog # MAB97791).

This product is intended for assay development on various assay platforms requiring antibody pairs.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Chikungunya Virus E1

Chikungunya fever is a pandemic disease caused by the flavivirus Chikungunya (CHIKV). The 47 kDa E1 glycoprotein mediates viral membrane fusion during CHIKV infection. The CHIKV envelope protein E1 is a component of the viral spike, which is composed of triplets of heterodimer of E1 and E2 glycoproteins, expressed on the viral surface. The viral spike proteins facilitate attachment to cell surfaces and viral entry into the cells. The E1 envelope protein is a class II fusion protein that mediates low pH-triggered membrane fusion during virus infection.

Long Name

Chikungunya Virus Envelope 1

Alternate Names

CHICKV E1, CHIKV-E1

Entrez Gene IDs

956308 (Viral)

Gene Symbol

CHIKVGP2

UniProt

Q8JUX5

Additional Chikungunya Virus E1 Products

Product Documents for Viral Chikungunya Virus E1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Viral Chikungunya Virus E1 Antibody

For research use only

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