Viral Dengue Virus 1 NS1 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB94381

R&D Systems, part of Bio-Techne
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MAB94381-100
MAB94381-SP
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Key Product Details

Species Reactivity

Viral

Applications

ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 973604

View all Dengue Virus 1 NS1 Antibodies »

Product Specifications

Immunogen

Human embryonic kidney cell line HEK293-derived viral Dengue Virus 1 NS1 protein
Asp776-Ala1127
Accession # P17763

Specificity

Detects viral Dengue Virus 1 NS1 protein in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Viral Dengue Virus 1 NS1 Antibody

Viral Dengue Virus 1 NS1 Antibody in ELISA Standard Curve.

Viral Dengue Virus 1 NS1 ELISA Standard Curve.

Recombinant Viral Dengue Virus 1 NS1 protein was serially diluted 2-fold and captured by Mouse Anti-Viral Dengue Virus 1 NS1 Monoclonal Antibody (Catalog # MAB9438) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Viral Dengue Virus 1 NS1 Monoclonal Antibody (Catalog # MAB94381) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Applications for Viral Dengue Virus 1 NS1 Antibody

Application
Recommended Usage

ELISA

This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Viral Dengue Virus 1 NS1 Monoclonal Antibody (Catalog # MAB9438).

This product is intended for assay development on various assay platforms requiring antibody pairs.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Dengue Virus 1 NS1

Dengue virus type 1 non-structural protein 1 (DENV1 NS1)  is an approximately 50 kDa viral glycoprotein (1). Dengue viruses are mosquito-borne flaviviruses that exist in nature as four distinct serotypes (DENV1-4) (2). Dengue virus NS1 can be found associated with cell membranes, within cells and at the cell surface, or as secreted extracellular species. The secreted form of NS1 is known to be hexameric (1). Within cells, NS1 plays a significant role in virus replication and has been shown to co-localize with dsRNA and other components of viral replication complexes in viral-induced vesicle packets (1,3). Cell surface-associated and secreted NS1 are profoundly immunogenic, and both the protein itself and the antibodies it elicits have been shown to contribute to either protection or pathogenesis and innate immune evasion (1, 3). Mature DENV1 NS1 contains 352 amino acids (aa) and consists of 2 conserved N-glycosylation sites and 12 invariant cysteine residues that form six intramolecular disulfide bonds (3).Mature DENV1 NS1 shares 73%, 80%, and 69% aa sequence identity with DENV2, DENV3, and DENV4 NS1, respectively.

References

    1. Muller, D. et al. (2012) J. Gen. Virol. 93:771.
    2. VanBlargan, L.A. et al. (2013) PLoS Pathog. 9:e1003761.
    3. Flamand, M. et al. (1999) J. Virol. 73:6104.

Long Name

Dengue Virus 1 Nauru/West Pac/1974 Polyprotein

Alternate Names

DEN-1, Dengue virus Serotype 1, Dengue virus Type 1, DENV1, DENV1-NS1

Entrez Gene IDs

5075725 (Viral)

Gene Symbol

POLY

UniProt

P17763

Additional Dengue Virus 1 NS1 Products

Product Documents for Viral Dengue Virus 1 NS1 Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Viral Dengue Virus 1 NS1 Antibody

For research use only

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