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Viral HIV-1 Gag p24 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB11189

R&D Systems, part of Bio-Techne
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MAB11189-100
MAB11189-SP

Key Product Details

Species Reactivity

Viral

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 1042942

Product Specifications

Immunogen

E. coli-derived recombinant viral HIV-1 Gag p24
Pro149-Gly354
Accession # Q77YG1_9HIV1

Specificity

Detects viral HIV-1 Gag p24 in direct ELISAs

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Viral HIV-1 Gag p24 Antibody

Detection of Recombinant HIV-1 Gag p24 HIV-1 Gag p24 by Western Blot.

Western blot shows lysates of rHIV-1 Gag p24. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Viral HIV-1 Gag p24 Monoclonal Antibody (Catalog # MAB11189) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). A specific band was detected for HIV-1 Gag p24 at approximately 23 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Applications for Viral HIV-1 Gag p24 Antibody

Application
Recommended Usage

ELISA

This antibody functions as an ELISA capture antibody when paired with mouse anti-HIV‑1 Gag p24 Monoclonal Antibody (Catalog # MAB11188).This product is intended for assay development on various assay platforms requiring antibody pairs.

Western Blot

1 µg/mL
Sample: rHIV-1 Gag p24

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HIV-1 Gag p24

The gag gene of human immunodeficiency virus 1 (HIV-1) encodes a precursor protein known as Pr55Gag. The viral protease PR cleaves this precursor to generate p17, p24, p7, and p6 proteins which are required for virus particle assembly. p24 is a major viral core structural protein. Its measurement is commonly used as an indicator of HIV-1 infection and viral load.

Long Name

Human Immunodeficiency Virus Type 1 GAG/Group-specific Antigen core protein of 24 kDa

Alternate Names

CA, Capsid Protein, HIV1 Gag p24, Pr55(Gag)

Entrez Gene IDs

155030 (Viral)

Gene Symbol

GAG

UniProt

Additional HIV-1 Gag p24 Products

Product Documents for Viral HIV-1 Gag p24 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Viral HIV-1 Gag p24 Antibody

For research use only

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