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Virilizer Antibody (HL1719) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-25744

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-25744

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL1719

Format

Azide and BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Summary for Virilizer Antibody (HL1719) - Azide and BSA Free

Immunogen

Recombinant protein encompassing a sequence within the center region of human Virilizer. The exact sequence is proprietary.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Virilizer Antibody (HL1719) - Azide and BSA Free

Virilizer Antibody (HL1719) - Azide and BSA Free

Western Blot: Virilizer Antibody (HL1719) - Azide and BSA Free [NBP3-25744] -

Western Blot: Virilizer Antibody (HL1719) - Azide and BSA Free [NBP3-25744] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with VIRMA antibody [HL1719] (NBP3-25744) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
Virilizer Antibody (HL1719) - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: Virilizer Antibody (HL1719) - Azide and BSA Free [NBP3-25744] -

Immunocytochemistry/Immunofluorescence: Virilizer Antibody (HL1719) - Azide and BSA Free [NBP3-25744] - VIRMA antibody [HL1719] detects VIRMA protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: VIRMA stained by VIRMA antibody [HL1719] (NBP3-25744) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.

Applications for Virilizer Antibody (HL1719) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Virilizer

The virilizer (vir) gene was discovered as a factor required for dosage compensation and sex determination in female flies. In drosophila it has been determined to function as a positive regulator of female-specific mRNA splicing. Database searches using the deduced amino acid sequence of the drosophila VIR retrieved a human protein BAA92667 (GenBank) which corresponds to the gene designated as KIAA1429. The human protein is 28% identical and about 40% similar to drosophila VIR and contains the putative transmembrane domain of drosophila VIR.

Alternate Names

DKFZp434I116, DKFZp781B2117, fSAP121, functional spliceosome-associated protein 121, KIAA1429, MGC138493, MGC141940, protein virilizer homolog

Gene Symbol

VIRMA

Additional Virilizer Products

Product Documents for Virilizer Antibody (HL1719) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Virilizer Antibody (HL1719) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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