visinin-like 1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-25168

Novus Biologicals, part of Bio-Techne
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NBP2-25168
NBP2-25168SS
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Porcine, Bovine

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Chicken IgY

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all VSNL1 Antibodies »

Product Specifications

Immunogen

Full length recombinant human VLP1

Clonality

Polyclonal

Host

Chicken

Isotype

IgY

Theoretical MW

22 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for visinin-like 1 Antibody

Western Blot: visinin-like 1 Antibody [NBP2-25168]

Western Blot: visinin-like 1 Antibody [NBP2-25168]

Western Blot: visinin-like 1 Antibody [NBP2-25168] - Analysis of different tissue lysates using chicken pAb to visinin-like protein 1 (VLP1), NBP2-25168, dilution 1:10,000 in green: [1] protein standard (red), [2] rat brain, [3] mouse brain, [4] pig hippocampus, and [5] cow cerebellum. The band at ~20kDa corresponds to VLP1 protein.
Immunohistochemistry: visinin-like 1 Antibody [NBP2-25168]

Immunohistochemistry: visinin-like 1 Antibody [NBP2-25168]

Immunohistochemistry: visinin-like 1 Antibody [NBP2-25168] - Confocal image of adult rat cerebellar cortex stained with NBP2-25168 (green), polyclonal antibody to NF-M: NB300-133 (red) and DNA (blue). NBP2-25168 reveals synapses in the molecular layer (ML) strongly. Synaptic regions are also seen in the granule cell layer (GC). The perikarya of Purkinje cells (PC) and dendrites and axons are revealed with NF-M antibody. Little staining of visinin-like 1 is seen in the white matter (WM).

Applications for visinin-like 1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:1000-1:2000

Immunohistochemistry

1:1000-1:2000

Western Blot

1:5000-1:10000
Application Notes
This visinin-like 1 antibody is useful for Immunocytochemistry/Immunofluorescence and Western Blot, where a band can be seen at ~22 kDa.

Formulation, Preparation, and Storage

Purification

IgY purified

Formulation

Supplied as concentrated total IgY preparation from egg yolk. Exact concentration of target specific IgY is not quantifiable as the preparation contains both immune IgY specific for the target and also irrelevant, non-immune IgY.

Preservative

0.035% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: VSNL1

Visinin was originally isolated biochemically from chicken retina as a major protein of about 24 kDa on SDS-PAGE. Following cloning and sequencing of visinin, several visinin-like proteins were discovered by homology screening. One of these, visinin-like 1 is a small calcium binding protein which is very abundant in the nervous system and is found only in neurons, though different neurons have different levels of expression. It is particularly concentrated in cerebellar Purkinje cells, and tends to be most abundant in perikarya and dendrites. The protein was discovered independently by several groups and is therefore also sometimes known as hippocalcin-like protein 3, HLP3, HPCAL3, HUVISL1, VLP-1, VILIP and VILIP-1. The protein belongs to the large superfamily of calmodulin and paravalbumin type proteins which function by binding calcium ions. Calcium binding alters the conformation of these proteins and allow them to interact with other binding partners, the properties of which they may alter. Visinin-like 1 has four "EF hand" domains, which are negatively charged helix-turn-helix peptides that are responsible for calcium binding. Visinin-like 1 is 191 amino acids in size and has a molecular weight on SDS-PAGE of ~22kDa. The protein has recently been suggested to be a useful biomarker of Alzheimer's disease and traumatic brain injury.

Long Name

Visinin Like 1

Alternate Names

HPCAL3, HUVISL1, VILIP, VISL1

Entrez Gene IDs

7447 (Human)

Gene Symbol

VSNL1

OMIM

600817 (Human)

UniProt

P62760 (Human)

Additional VSNL1 Products

Product Documents for visinin-like 1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for visinin-like 1 Antibody

Chicken products cannot be exported to Canada.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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